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Journal of Assisted Reproduction and Genetics

, Volume 14, Issue 10, pp 603–608 | Cite as

Antiphospholipid Antibodies Associated with Implantation Failure After IVF/ET

  • Carolyn B. Coulam
  • Brian D. Kaider
  • Azadeth S. Kaider
  • Patrick Janowicz
  • Roumen G. Roussev
Article

Abstract

Purpose: Our purpose was to determine the specific anti-phospholipid antibodies (APAs) that should be evaluated to identify individuals at risk for implantation failure associated with reproductive autoimmune failure syndrome (RAFS).

Methods: The prevalence of APAs among 312 women with implantation failure was compared with that of 100 fertile control women. To be included in the implantation failure group, each woman had to have had at least 12 embryos transferred without subsequent positive pregnancy test. Enzyme-linked immunoabsorbant assay was used to measure IgG, IgM, and IgA anticardiolipin, antiphosphatidyl ethanol-amine, antiphosphatidyl inositol, antiphospatidic acid, antiphosphatidyl glycerol, antiphosphatidyl choline, and antiphosphatidyl serine.

Results: When the values for each of the seven APAs in three isotypes were compared between women with implantation failure and the control population, all of the APAs tested had a significantly higher frequency among women with implantation failure. Positive APAs were detected in 69 (22%) of the 312 women with implantation failure compared with 5 (5%) of the 100 control women (P < 0.0001). Anticardiolipin antibodies were found in 13 (4%) of the 312 women with implantation failure and none of the controls. Fifty-six (18%) of the 312 with implantation failure were negative for anticardiolipin antibodies but had positive values of other APAs.

Conclusions: A complete APA panel using seven isotypes is necessary for diagnosing implantation failure associated with RAFS. If only anticardiolipin antibody is measured, 4% (13/312) of the positive APAs are detected, and 81% (56/69) of women with implantation failure associated with RAFS will have the diagnosis missed.

antiphospholipid antibodies implantation failure in vitro fertilization embryo transfer 

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Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • Carolyn B. Coulam
    • 1
  • Brian D. Kaider
    • 1
  • Azadeth S. Kaider
    • 1
  • Patrick Janowicz
    • 1
  • Roumen G. Roussev
    • 1
  1. 1.The Center for Human ReproductionChicago

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