Isolation and characterization of a new keratinolytic Bacillus licheniformis strain

  • L. Manczinger
  • M. Rozs
  • Cs. Vágvölgyi
  • F. Kevei

Abstract

A keratin-degrading bacterium strain (K-508) was isolated from partially degraded feathers and characterized. This isolate exhibited a high chicken feather-degrading activity when cultured in feather-containing broth with a growth optimum of pH 7.0 and 47 °C. On the basis of its phenotypic characteristics (quickly moving, Gram-positive rods), the results of metabolic tests and rDNA sequence analysis, it was identified as Bacillus licheniformis. Its fermentation broth showed activity on N-Bz-l-Phe-l-Val-l-Arg-p-nitroanilide, N-Suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide, N-CBZ-Gly-Gly-l-Leu-p-nitroanilide and N-CBZ-l-Ala-l-Ala-l-Leu-p-nitroanilide as chromogenic protease substrates at near neutral pH. Both trypsin-like and chymotrypsin-like proteases were constitutively secreted by this strain.

Bacillus licheniformis chymotrypsin-like protease trypsin-like protease 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • L. Manczinger
    • 1
  • M. Rozs
    • 1
  • Cs. Vágvölgyi
    • 1
  • F. Kevei
    • 1
  1. 1.Faculty of Sciences, Department of MicrobiologyUniversity of Szeged

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