Plant Molecular Biology

, Volume 51, Issue 5, pp 703–718 | Cite as

Identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana

  • Hans van Leeuwen
  • Amparo Monfort
  • Hong-Bin Zhang
  • Pere Puigdomènech


A bacterial artificial chromosome (BAC) library from the dihaploid melon line `PIT92' was constructed with a 6 times coverage of the haploid melon genome. A contig of four BACs around the MRGH63 resistance gene homologue fragment was created. The complete sequence of a 117-kb BAC clone allowed to determine two clearly defined regions, the first one containing a cluster of three resistance gene homologues. Separated by a retrotransposon, that contains large long terminal repeats, the second region presents a group of genes with a conserved distribution in two regions of the Arabidopsis genome. The detailed analysis of this region provides a description of the gene structure and the presence of repetitive sequences in a defined fragment of the genome of Cucumis melo.

Bacterial Artificial Chromosome colinearity Cucurbitaceae genome organisation simple sequence repeats and retrotransposons synteny 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Hans van Leeuwen
    • 1
  • Amparo Monfort
    • 1
    • 2
  • Hong-Bin Zhang
    • 3
  • Pere Puigdomènech
    • 1
  1. 1.Departament de Genètica MolecularInstitut de Biologia Molecular de Barcelona, CID-CSICBarcelonaSpain
  2. 2.Departament de Genètica VegetalIRTA, Ctra. de Cabrils s/nCabrils, BarcelonaSpain
  3. 3.Department of Soil and Crop Sciences and Institute for Plant Genomics and Biotechnology, 2123 TAMUTexas A&M UniversityCollege StationUSA

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