Advertisement

Plant Growth Regulation

, Volume 39, Issue 2, pp 125–130 | Cite as

Quantification of endogenous gibberellins in leaves and tubers of Chinese yam, Dioscorea opposita Thunb. cv. Tsukune during tuber enlargement

  • Sang-Kuk Kim
  • Sang-Chul Lee
  • Dong-Hyun Shin
  • Soo-Won Jang
  • Jae-Won Nam
  • Tae-Shik Park
  • In-Jung Lee
Article

Abstract

Elevengibberellins (GAs) were identified and quantified in extracts of leaves andtubers of the Chinese yam, Dioscorea opposita Thunb. cv.Tsukune by GC-MS-SIM and Kovats retention indices. Five of these gibberellinsare members of the early-13-hydroxylation pathway (GA53,GA44, GA19, GA20 and GA1), and sixare members of the non-13-hydroxylation pathway (GA12,GA15, GA24, GA9, GA36 andGA4). Of these eleven, GA44, GA15 andGA1 were detected for the first time in Dioscoreaopposita leaf tissues. The major biosynthetic GA pathway in leavesofChinese yam was non C-13 hydroxylation (NCH). In addition, the activeGA4 content for all harvest dates was greater than that ofGA1 in the leaves and tubers during tuber development. It issuggested that the higher level of GA4 in the leaves and tubers maybe closely related to tuber enlargement.

Dioscorea opposita Thunb GC-MS-SIM Gibberellins Quantification 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Boother G.M., Gale M.D., Gaskin P., MacMillan J. and Sponsel V.M. 1991. Gibberellins in shoots of Hordeum vulgare. A comparison between cv. Triumph and two dwarf mutants which differ in their response to gibberellin. Physol. Plant 81: 385–392.Google Scholar
  2. Ewing E.E. 1987. The role of hormones in potato (Solanum tuberosumL.) tuberisation. In: Davies P.J. (ed.), Plant Hormones and Their Role in Plant Growth and Development. Martinus Nijhoff Publishers, Boston, pp. 515-538.Google Scholar
  3. Gaskin P. and MacMillan J. 1991. GC-MS of Gibberellins and Related Compounds: Methodology and a Library of Reference Spectra. Cantocks Enterprises, Bristol, UK.Google Scholar
  4. GuiñazÚ M., Abdala G. and Tizio R. 1988. Effect of free and conjugated gibberellins on roots of potato cuttings treated with CCC in relation to tuber formation. J. Plant Physiol. 132: 725–730.Google Scholar
  5. Koda Y. and Okazawa Y. 1983. Characteristic changes in levels of endogenous plant hormones in relation to the onset of potato tuberisation. Jpn. J. Crop Sci. 52: 592-597.Google Scholar
  6. Koda Y. and Kikuta Y. 1991. Possible involvement of jasmonic acid in tuberisation of yam plants. Plant Cell Physiol. 32: 629-633.Google Scholar
  7. Kumar D. and Wareing P.F. 1972. Factors controlling stolon development in the potato plant. New Phytol. 71: 639-648.Google Scholar
  8. Lee I.J., Foster K.R. and Morgan P.W. 1998. Photoperiod control of gibberellin levels and flowering in Sorghum. Plant Physiol. 116: 1003-1011.Google Scholar
  9. Okagami N. 1972. The nature of gibberellin-induced dormancy in aerial tubers of Begonia evansiana. Plant Cell Physiol. 13: 763–771.Google Scholar
  10. Okagami N. and Nagao M. 1971. Gibberellin-induced dormancy in bulbils of Dioscorea. Planta 101: 91-94.Google Scholar
  11. Okagami N. and Tanno N. 1977. Dormancy in Dioscorea:generality of gibberellin induced dormancy in asexual dormant organs. Plant Cell Physiol. 18: 309-316.Google Scholar
  12. Sponsel V.M. 1995. Gibberellin biosynthesis and metabolism. In: Davies P.J. (ed.), Plant Hormones: Physiology, Biochemistry and Molecular Biology. Kluwer, Dordrecht, The Netherlands, pp. 66-97.Google Scholar
  13. Tanno N., Yokota T., Abe M. and Okagami N. 1992. Identification of endogenous gibberellins in dormant bulbils of Chinese yam, Dioscorea opposita. Plant Physiol. 100: 1823-1826.Google Scholar
  14. Vreugdenhil D. and Struik P.C. 1989. An integrated view of the hormonal regulation of tuber formation in potato (Solanum tuberosum). Physiol. Plantarum 75: 525-531.Google Scholar
  15. Xu X., van Lammeren A.A.M. and Vreugdenhil D. 1998. The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiol. 117: 575-584.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Sang-Kuk Kim
    • 1
  • Sang-Chul Lee
    • 2
  • Dong-Hyun Shin
    • 2
  • Soo-Won Jang
    • 2
  • Jae-Won Nam
    • 2
  • Tae-Shik Park
    • 1
  • In-Jung Lee
    • 2
  1. 1.Institute of Bioresources, Kyongbuk Provincial Agricultural Technology AdministrationAndongThe Republic of Korea
  2. 2.Division of Plant BiosciencesKyungpook National UniversityDaeguThe Republic of Korea

Personalised recommendations