Plant Molecular Biology

, Volume 51, Issue 5, pp 719–729 | Cite as

The Arabidopsis thaliana homeobox gene ATHB5 is a potential regulator of abscisic acid responsiveness in developing seedlings

  • Henrik Johannesson
  • Yan Wang
  • Johannes Hanson
  • Peter Engström


ATHB5 is a member of the homeodomain-leucine zipper (HDZip) transcription factor gene family of Arabidopsis thaliana. In this report we show that increased expression levels of ATHB5 in transgenic Arabidopsis plants cause an enhanced sensitivity to the inhibitory effect of abscisic acid (ABA) on seed germination and seedling growth. Consistent with this finding we demonstrate in northern blot experiments that the ABA-responsive gene RAB18 is hyperinduced by ABA in transgenic overexpressor lines as compared to the wild type. Northern blot and promoter-GUS fusion analyses show that ATHB5 gene transcription is initiated rapidly after the onset of germination and localized primarily to the hypocotyl of germinating seedlings. Moreover, analysis of ATHB5 gene expression during post-germinative growth in different ABA response mutants shows that ATHB5 gene activity is down-regulated in the abi1-1, abi3-1 and abi5-1 mutant lines, but not in abi2-1 or abi4-1. The identification of a T-DNA insertion mutant line of ATHB5 is described and no phenotypic alterations could be discerned, suggesting that ATHB5 may act redundantly with other HDZip genes. Taken together, these data suggest that ATHB5 is a positive regulator of ABA-responsiveness, mediating the inhibitory effect of ABA on growth during seedling establishment.

abscisic acid Arabidopsis germination homeobox plant seedling establishment 


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  1. Bechtold, N. and Pelletier, G. 1998. In planta Agrobacteriummediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration. Meth. Mol. Biol. 82: 259–266.Google Scholar
  2. Bewley, J.D. and Black, M. 1994. Seeds: Physiology of Development and Germination. Plenum, New York.Google Scholar
  3. Cutler, S., Ghassemian, M., Bonetta, D., Cooney, S. and McCourt, P. 1996. A protein farnesyl transferase involved in abscisic acid signal transduction in Arabidopsis. Science 273: 1239–1241.Google Scholar
  4. Elomaa, P., Honkanen, J., Puska, R., Seppänen, P., Helariutta, Y., Mehto, M., Kotilainen, M., Nevalainen, L. and Teeri, T.H. 1993. Agrobacterium-mediated transfer of antisense chalcone synthase cDNA to Gerbera hybrida inhibits flower pigmentation. Bio/technology 11: 508–511.Google Scholar
  5. Finkelstein, R.R. 1994. Mutations at two new Arabidopsis ABA response loci are similar to the abi3 mutations. Plant J. 5: 765–771.Google Scholar
  6. Finkelstein, R.R. and Lynch, T.J. 2000. The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor. Plant Cell 12: 599–609.Google Scholar
  7. Finkelstein, R.R. and Somerville, C.R. 1990. Three classes of abscisic acid (ABA)-insensitive mutations of Arabidopsis define genes that control overlapping subsets of ABA responses. Plant Physiol. 94: 1172–1179.Google Scholar
  8. Finkelstein, R.R., Wang, M.L., Lynch, T.J., Rao, S. and Goodman, H.M. 1998. The Arabidopsis abscisic acid response locus ABI4 encodes an APETALA 2 domain protein. Plant Cell 10: 1043–1054.Google Scholar
  9. Garciarrubio, A., Legaria, J.P. and Covarrubias, A.A. 1997. Abscisic acid inhibits germination of mature Arabidopsis seeds by limiting the availability of energy and nutrients. Planta 203: 182–187.Google Scholar
  10. Giraudat, J., Hauge, B.M., Valon, C., Smalle, J., Parcy, F. and Goodman, H.M. 1992. Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell 4: 1251–1261.Google Scholar
  11. Himmelbach, A., Iten, M. and Grill, E. 1998. Signalling of abscisic acid to regulate plant growth. Phil. Trans. R. Soc. London B Biol. Sci. 353: 1439–1444.Google Scholar
  12. Johannesson, H., Wang, Y. and Engström, P. 2001. DNA-binding and dimerization preferences of Arabidopsis homeodomainleucine zipper transcription factors in vitro. Plant Mol. Biol. 45: 63–73.Google Scholar
  13. Koornneef, M., Reuling, G. and Karssen, C.M. 1984. The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana. Physiol. Plant 61: 377–383.Google Scholar
  14. Lång, V. and Palva, E.T. 1992. The expression of a rab-related gene, rab18, is induced by abscisic acid during the cold acclimation process of Arabidopsis thaliana (L.) Heynh. Plant Mol. Biol. 20: 951–962.Google Scholar
  15. Lee, Y.H. and Chun, J.Y. 1998. A new homeodomain-leucine zipper gene from Arabidopsis thaliana induced by water stress and abscisic acid treatment. Plant Mol. Biol. 37: 377–384.Google Scholar
  16. Leung, J. and Giraudat, J. 1998. Abscisic acid signal transduction. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49: 199–222.Google Scholar
  17. Leung, J., Bouvier-Durand, M., Morris, P.C., Guerrier, D., Chefdor, F. and Giraudat, J. 1994. Arabidopsis ABA response gene ABI1: features of a calcium-modulated protein phosphatase. Science 264: 1448–1452.Google Scholar
  18. Leung, J., Merlot, S. and Giraudat, J. 1997. The Arabidopsis ABSCISIC ACID INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. Plant Cell 9: 759–771.Google Scholar
  19. Lin, Y. and Schiefelbein, J. 2001. Embryonic control of epidermal cell patterning in the root and hypocotyl of Arabidopsis. Development 128: 3697–3705.Google Scholar
  20. Lopez-Molina, L., Mongrand, S. and Chua, N.H. 2001. A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis. Proc. Natl. Acad. Sci. USA 98: 4782–4787.Google Scholar
  21. Malamy, J.E. and Benfey, P.N. 1997. Organization and cell differentiation in lateral roots of Arabidopsis thaliana. Development 124: 33–44.Google Scholar
  22. Meyer, K., Leube, M.P. and Grill, E. 1994. A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana. Science 264: 1452–1455.Google Scholar
  23. Nakamura, S., Lynch, T.J. and Finkelstein, R.R. 2001. Physical interactions between ABA response loci of Arabidopsis. Plant J. 26: 627–635.Google Scholar
  24. Pei, Z.M., Ghassemian, M., Kwak, C.M., McCourt, P. and Schroeder, J.I. 1998. Role of farnesyl transferase in ABA regulation of guard cell anion channels and plant water loss. Science 282: 287–290.Google Scholar
  25. Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Plainview, NY.Google Scholar
  26. Sessa, G., Morelli, G. and Ruberti, I. 1993. The Athb-1 and-2 HDZip domains homodimerize forming complexes of different DNA binding specificities. EMBO J. 12: 3507–3517.Google Scholar
  27. Söderman, E., Hjellstrom, M., Fahleson, J. and Engström, P. 1999. The HD-Zip gene ATHB6 in Arabidopsis is expressed in developing leaves, roots and carpels and up-regulated by water deficit conditions. Plant Mol. Biol. 40: 1073–1083.Google Scholar
  28. Söderman, E., Mattsson, J., Svenson, M., Borkird, C. and Engström, P. 1994. Expression patterns of novel genes encoding homeodomain leucine-zipper proteins in Arabidopsis thaliana. Plant Mol. Biol. 26: 145–154.Google Scholar
  29. Söderman, E., Mattsson, J. and Engström, P. 1996. The Arabidopsis homeobox gene ATHB-7 is induced by water deficit and by abscisic acid. Plant J. 10: 375–381.Google Scholar
  30. Söderman, E.M., Brocard, I.M., Lynch, T.J. and Finkelstein, R.R. 2000. Regulation and function of the Arabidopsis ABAinsensitive4 gene in seed and abscisic acid response signalling networks. Plant Physiol. 124: 1752–1765.Google Scholar
  31. Verwoerd, T.C., Dekker, B.M. and Hoekema, A. 1989. A smallscale procedure for the rapid isolation of plant RNAs. Nucl. Acids Res. 17: 2362.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Henrik Johannesson
    • 1
  • Yan Wang
    • 1
  • Johannes Hanson
    • 1
  • Peter Engström
    • 1
  1. 1.Department of Physiological BotanyEvolutionary Biology CenterUppsalaSweden e-mail

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