Cellular and Molecular Neurobiology

, Volume 18, Issue 2, pp 231–247 | Cite as

Multifactorial Modulation of TRH Metabolism

  • P. Joseph-Bravo
  • R. M. Uribe
  • M. A. Vargas
  • L. Pérez-Martínez
  • T. Zoeller
  • J. L. Charli


1. Thyrotropin releasing hormone (TRH), synthesized in the paraventricular nucleus of the hypothalamus (PVN), is released in response to physiological stimuli through medianeminence nerve terminals to control thyrotropin or prolactin secretion from the pituitary.

2. Several events participate in the metabolism of this neuropeptide: regulation of TRH biosynthesis and release as well as modulation of its inactivation by the target cell.

3. Upon a physiological stimulus such as cold stress or suckling, TRH is released and levels of TRH mRNA increase in a fast and transient manner in the PVN; a concomitant increase in cfos is observed only with cold exposure.

4. Hypothalamic cell cultures incubated with cAMP or phorbol esters show a rise in TRH mRNA levels; dexamethasone produces a further increase at short incubation times.TRH mRNA are thus controlled by transsynaptic and hormonal influences.

5. Once TRH is released, it is inactivated by a narrow specificity ectoenzyme, pyroglu-tamyl peptidase II (PPII).

6. In adenohypophysis, PPII is subject to stringent control: positive by thyroid hormones and negative by TRH; other hypothalamic factors such as dopamine and somatostatin also influence its activity.

7. These combined approaches suggest that TRH action is modulated in a coordinate fashion.

thyrotropin releasing hormone (TRH) pryoglutamyl peptidase II paraventricular nucleus lactotroph convertases PC1 PC2 TRH mRNA neuroendocrine regulation glucocorticoids thyroid hormones suckling cold 


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Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • P. Joseph-Bravo
    • 1
    • 2
  • R. M. Uribe
    • 1
  • M. A. Vargas
    • 1
  • L. Pérez-Martínez
    • 1
  • T. Zoeller
    • 3
  • J. L. Charli
    • 1
  1. 1.Department of Molecular Genetics and Physiology, Institute of BiotechnologyUniversidad Nacional Autonoma de MéxicoCuernavacaMéxico
  2. 2.Instituto de Biotecnología, UNAMCuernavacaMexico
  3. 3.Department of BiologyUniversity of MassachusettsAmherst

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