Molecular Breeding

, Volume 11, Issue 2, pp 159–168

In vitro regeneration and transformation of pigeonpea [Cajanus cajan (L.) Millsp]

  • Tran Thanh Thu
  • Tran Thi Xuan Mai
  • Eric Dewaele
  • Slama Farsi
  • Yohannes Tadesse
  • Geert Angenon
  • Michel Jacobs
Article

DOI: 10.1023/A:1022497811702

Cite this article as:
Thu, T.T., Xuan Mai, T.T., Dewaele, E. et al. Molecular Breeding (2003) 11: 159. doi:10.1023/A:1022497811702

Abstract

A requirement for generating transgenic pigeonpea [Cajanuscajan (L.) Millsp] plants is the development of a highly efficientin vitro regeneration procedure. This goal was achieved byusing germinated seedlings grown on B5 medium supplemented with 10 mgl−1 6-benzylaminopurine, which induced differentiatingcallus formation in the cotyledonary node region. The calli were transferred onB5 medium with 0.2 mg l−1 6-benzylaminopurine toobtain shoot induction. Elongated shoots were then further cultured on a B5hormone-free medium for rooting. Using this regeneration system transgenicpigeonpea plants were obtained both by particle bombardment andAgrobacterium tumefaciens-mediated gene transfer. Thepresence of the transgenes in the pigeonpea genome was confirmed by GUS assays,PCR and Southern hybridisation. The transgenic rooted plants were successfullytransferred to soil in the greenhouse. GUS and PCR assays of T1 progeniesconfirmed that the transgenes were stably transmitted to the next generation.This is the first report of successful use ofAgrobacteriumas well as particle bombardment for production of transgenic pigeonpea plants.

Agrobacterium Particle bombardment Pigeonpea Plant regeneration Transgenic plants 

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Tran Thanh Thu
    • 1
  • Tran Thi Xuan Mai
    • 1
  • Eric Dewaele
    • 1
  • Slama Farsi
    • 1
  • Yohannes Tadesse
    • 1
  • Geert Angenon
    • 1
  • Michel Jacobs
    • 1
  1. 1.Laboratory of Plant Genetics, Institute of Molecular Biology and BiotechnologyVrije Universiteit BrusselSint-Genesius-RodeBelgium

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