Neurochemical Research

, Volume 23, Issue 4, pp 455–461 | Cite as

Manda, a Fermented Natural Food, Suppresses Lipid Peroxidation in the Senescent Rat Brain

  • Motoko Kawai
  • Shingoro Matsuura
  • Masato Asanuma
  • Norio Ogawa


The level of lipid peroxidation reflects the degree of free radical-induced oxidative damage in brain tissue of the elderly. We examined the effects of Manda, a product prepared by yeast fermentation of several fruits and black sugar, on lipid peroxidation in the senescent rat brain as model of aging. Senescent rats were provided with a diet containing 50 g/100 g Manda for 8 days, supplemented on day 8 with an intragastric administration of Manda (6.0 g/kg body wt.) twice daily. The hydroxyl radical scavenging activity was generated by the FeSO4-H2O2 system and analyzed by electron spin resonance spectrometry. Using this method, the addition of Manda (2.88 mg/ml) to brain homogenates of adult rats (0.06 mg/ml) had an additive inhibitory effect on lipid peroxidation compared with control adult rats not treated with Manda. Incubation of brain homogenates with Manda for 2 h and 3 h, significantly inhibited the increase in lipid peroxides (malondialdehydes and 4-hydroxyalkenals) levels in aged rats due to auto-oxidation. In addition, oral administration of Manda significantly suppressed the age-related increase in lipid peroxidation in the hippocampus and striatum, although such change was not observed in the cerebral cortex. Although Manda contains trace level of α-tocopherol, the level of α-tocopherol in Manda did no correlate with its antioxidant effect. Our results suggest that Manda protects against age-dependent oxidative neuronal damage caused by oxidative stress and that this protective effect may be due, in part, to its scavenging activity against free radicals.

Lipid peroxidation aging antioxidant brain Fischer rats 


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Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Motoko Kawai
    • 1
  • Shingoro Matsuura
    • 2
  • Masato Asanuma
    • 1
  • Norio Ogawa
    • 1
  1. 1.Department of Neuroscience, Institute of Molecular and Cellular MedicineOkayama University Medical SchoolOkayamaJapan
  2. 2.Manda Fermentation Co., Ltd.Innoshima-shi, HiroshimaJapan

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