Neurochemical Research

, Volume 23, Issue 6, pp 919–922 | Cite as

Elevated Nerve Growth Factor Levels in the Synovial Fluid of Patients with Inflammatory Joint Disease

  • Dale A. Halliday
  • Christian Zettler
  • Robert A. Rush
  • Raffaele Scicchitano
  • Julian D. McNeil


A novel pH shock extraction procedure was used to measure nerve growth factor (NGF) levels in both normal and inflamed synovial fluids using a sensitive and specific two-site enzyme linked immunosorbant assay. To date no data is available on NGF levels in normal synovial fluids. Synovial fluids were taken from 5 normal volunteers, 12 patients with rheumatoid arthritis and 10 patients with other inflammatory arthropathies. The mean ± SEM NGF concentration in normal synovial fluids was 95 ± 33.2 pg/ml (range 39.1–143.1 pg/ml), whereas the mean NGF concentration in the synovial fluids taken from patients with rheumatoid arthritis was 532.5 ± 123.8 pg/ml (range 152–1686 pg/ml). The mean NGF concentration in patients with other inflammatory arthropathies was also raised (430.6 ± 90 pg/ml; range 89–1071 pg/ml). The NGF concentrations were significantly higher in the synovial fluids from both inflamed groups (ANOVA p < 0.05) compared to normals. Raised levels of NGF in synovial fluid may contribute directly to joint inflammation via activation of inflammatory cells.

Nerve growth factor inflammation arthritis synovial fluid pain 


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Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Dale A. Halliday
    • 1
  • Christian Zettler
    • 2
  • Robert A. Rush
    • 2
  • Raffaele Scicchitano
    • 3
  • Julian D. McNeil
    • 4
  1. 1.Department of Immunopathology Women's and Children's HospitalAdelaideSouth Australia
  2. 2.Department of Physiology and Centre for NeuroscienceFlinders University of South AustraliaAdelaideSouth Australia
  3. 3.Department of Thoracic MedicineRoyal Adelaide Hospital, North TceAdelaideSouth Australia
  4. 4.Department of Medicine, Modbury HospitalThe University of Adelaide, North TceAdelaideSouth Australia

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