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Neurochemical Research

, Volume 23, Issue 3, pp 385–392 | Cite as

αB-Crystallin is Associated with Intermediate Filaments in Astrocytoma Cells

  • Thomas Wisniewski
  • James E. Goldman
Article

Abstract

αB-crystallin, a major protein of the vertebrate lens and a member of the small heat shock protein family, is expressed in non-lenticular tissues, including the central nervous system, where it is found mainly in glia. In Rosenthal fibers (RF), astrocytic inclusions that accumulate in Alexander's Disease, αB-crystallin is found with hsp27 and skeins of intermediate filaments (IF) of the GFAP and vimentin types. We have investigated the association between IF and αB-crystallin in a human astrocytoma cell line, U-373MG, which expresses αB-crystallin. Cytoskeletal preparations contained αB-crystallin, and a filamentous pattern in which αB-crystallin co-localized with GFAP and vimentin by double label immunofluorescence. Immuno-electronmicroscopy confirmed the localization to IF. GFAP isolated from bovine brain and re-assembled, was associated with αB-crystallin. Thus, a proportion of αB-crystallin in astroglia is associated with IF, and this association may be critical in the formation of RF.

Alpha B-crystallin intermediate filaments astrocytes GFAP 

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Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Thomas Wisniewski
    • 1
  • James E. Goldman
    • 2
  1. 1.Department of NeurologyNew York University Medical CenterNew York
  2. 2.Department of Pathology, Division of Neuropathology, and the Center for Neurobiology and BehaviorColumbia University, College of Physicians and SurgeonsNew YorkUSA

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