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Plant Cell, Tissue and Organ Culture

, Volume 72, Issue 2, pp 147–151 | Cite as

The influence of animal sex hormones on the induction of flowering in Arabidopsis thaliana: comparison with the effect of 24-epibrassinolide

  • Anna JaneczkoEmail author
  • Władysław Filek
  • Jolanta Biesaga-Kościelniak
  • Izabela Marcińska
  • Zbigniew Janeczko
Article

Abstract

The influence of selected steroids on the in vitro generative development of Arabidopsis thalianawas investigated. The activity of the animal steroids androsterone, androstenedione, progesterone, estrone, estriol, and 17β-estradiol was compared to 24-epibrassinolide, a member of the regulatory family of brassinosteroids. A. thaliana plants were cultured in vitro in media containing these steroids. The stimulatory effect of the tested substances was evaluated by measurement of the percentage of generative plants versus vegetative plants in the experimental group. It was established that androstenedione, the main testosterone precursor, and androsterone, a typical male hormone, were more effective in stimulating flowering in A. thaliana than the female hormones, estrogens and progesterone. Androsterone at a concentration of 0.1 μM increased the percentage of generative plants up to 96% (control 41%). Estrogens at the same concentration decreased the number of generative plants and 24-epibrassinolide did not stimulate A. thalianagenerative development.

animal sex hormones Arabidposis thaliana induction of flowering in vitro cultures 24-epibrassinolide 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Anna Janeczko
    • 1
    • 2
    Email author
  • Władysław Filek
    • 2
  • Jolanta Biesaga-Kościelniak
    • 1
  • Izabela Marcińska
    • 1
  • Zbigniew Janeczko
    • 3
  1. 1.Department of Plant PhysiologyPolish Academy of ScienceCracowPoland
  2. 2.Chair of Plant Physiology, Faculty of AgricultureAgricultural University in CracowCracowPoland
  3. 3.Department of Pharmacognosy College of MedicineJagiellonian UniversityCracowPoland

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