Euphytica

, Volume 129, Issue 3, pp 319–323

Obtaining an Ogura-type CMS line from asymmetrical protoplast fusion between cabbage (fertile) and radish (fertile)

  • Takeshi Motegi
  • Ill Sup Nou
  • Jinmei Zhou
  • Akira Kanno
  • Toshiaki Kameya
  • Yutaka Hirata
Article

Abstract

Cytoplasmic male sterile (CMS) plants were obtained by asymmetrical protoplast fusion between red cabbage (fertile) and normal radish (fertile). The CMS plants showed restriction fragment length polymorphism (RFLP) patterns of mitochondrial (mt) DNA that were different from those of both parental lines. PCR analysis of mtDNA of the CMS plants and though Southern blot analysis showed that the mtDNA of the CMS line had the characteristics of the ogura CMS type. The results suggested that the orf138 gene and the ogura type atp6 gene are present in normal fertile cabbage and radish at a low copy level.

mitochondrial DNA ogura CMS cytoplasm somatic hybridization stoichiometric shift 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Takeshi Motegi
    • 1
  • Ill Sup Nou
    • 2
  • Jinmei Zhou
    • 1
  • Akira Kanno
    • 3
  • Toshiaki Kameya
    • 3
  • Yutaka Hirata
    • 1
  1. 1.Faculty of Agriculture, TokyoUniversity of Agriculture and TechnologyFuchu, TokyoJapan
  2. 2.School of Plant Science, College of Agriculture and Life ScienceSunchon UniversitySunchon, Chonnam-DoKorea
  3. 3.Institute of Genetic EcologyTohoku UniversityAmamiya-cho, SendaiJapan

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