Obtaining an Ogura-type CMS line from asymmetrical protoplast fusion between cabbage (fertile) and radish (fertile)
Cytoplasmic male sterile (CMS) plants were obtained by asymmetrical protoplast fusion between red cabbage (fertile) and normal radish (fertile). The CMS plants showed restriction fragment length polymorphism (RFLP) patterns of mitochondrial (mt) DNA that were different from those of both parental lines. PCR analysis of mtDNA of the CMS plants and though Southern blot analysis showed that the mtDNA of the CMS line had the characteristics of the ogura CMS type. The results suggested that the orf138 gene and the ogura type atp6 gene are present in normal fertile cabbage and radish at a low copy level.
Unable to display preview. Download preview PDF.
- Aldrich, J. & C.A. Cullis, 1993. RAPD analysis in flax: Optimization of yield and reproducibility using KlenTaq 1 DNA polymerase, Chelex 100, and gel purification of genomic DNA. Plant Mol Biol Rep 11: 128-141.Google Scholar
- Fu, T.D., 1981. Production and research of rapeseed in the People's Republic of China. Eucarpia Cruciferae Newsletter 6: 6-7.Google Scholar
- Kameya, T., H. Kanzaki, S. Toki & T. Abe, 1989. Transfer of radish (Raphanus sativus L.) chloroplasts into cabbage (Brassica oleracea L.) by protoplast fusion. Jpn J Genet 64: 27-34.Google Scholar
- Kanno, A., H. Kanzaki & T. Kameya, 1997. Detailed analysis of chloroplast and mitochondrial DNAs from the hybrid plant generated by asymmetric protoplast fusion between radish and cabbage. Plant Cell Rep 16: 479-484.Google Scholar
- Ogura, H., 1968. Studies on the new male sterility in Japanese radish, with special references to the utilization of this sterility towards practical raising of hybrid seed. Mem Fac Agric Kagoshima Univ 6: 39-78.Google Scholar
- Shiga, T., 1980. Male sterility and cytoplasmic differentiation. In: Brassica Crops and Wild Allies-Biology and breeding, Chap. 12, pp. 205-221. Japan Sci Soc Press, Tokyo.Google Scholar
- Thompson, K.F., 1972. Cytoplasmic male sterility in oilseed rape. Heredity 29: 253-257.Google Scholar
- Umbeck, P.F. & B.G. Gengenbach, 1983. Reversion of male-sterile T-cytoplasm maize to male fertility in tissue culture. Crop Sci 23: 384-388.Google Scholar