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Obtaining an Ogura-type CMS line from asymmetrical protoplast fusion between cabbage (fertile) and radish (fertile)

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Abstract

Cytoplasmic male sterile (CMS) plants were obtained by asymmetrical protoplast fusion between red cabbage (fertile) and normal radish (fertile). The CMS plants showed restriction fragment length polymorphism (RFLP) patterns of mitochondrial (mt) DNA that were different from those of both parental lines. PCR analysis of mtDNA of the CMS plants and though Southern blot analysis showed that the mtDNA of the CMS line had the characteristics of the ogura CMS type. The results suggested that the orf138 gene and the ogura type atp6 gene are present in normal fertile cabbage and radish at a low copy level.

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Authors and Affiliations

  1. Faculty of Agriculture, Tokyo, University of Agriculture and Technology, Fuchu, Tokyo, Japan

    Takeshi Motegi, Jinmei Zhou & Yutaka Hirata

  2. School of Plant Science, College of Agriculture and Life Science, Sunchon University, Sunchon, Chonnam-Do, Korea

    Ill Sup Nou

  3. Institute of Genetic Ecology, Tohoku University, Amamiya-cho, Sendai, Japan

    Akira Kanno & Toshiaki Kameya

Authors
  1. Takeshi Motegi
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  2. Ill Sup Nou
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  3. Jinmei Zhou
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  4. Akira Kanno
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  5. Toshiaki Kameya
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  6. Yutaka Hirata
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Motegi, T., Sup Nou, I., Zhou, J. et al. Obtaining an Ogura-type CMS line from asymmetrical protoplast fusion between cabbage (fertile) and radish (fertile). Euphytica 129, 319–323 (2003). https://doi.org/10.1023/A:1022284803689

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