Glycoconjugate Journal

, Volume 18, Issue 11–12, pp 907–914

Abnormal subcellular distribution of mature MUC2 and de novo MUC5AC mucins in adenomas of the rectum: Immunohistochemical detection using non-VNTR antibodies to MUC2 and MUC5AC peptide

  • Neil Myerscough
  • Paul A. Sylvester
  • Bryan F. Warren
  • Simon Biddolph
  • Paul Durdey
  • Michael G. Thomas
  • Ingemar Carlstedt
  • Anthony P. Corfield
Article

DOI: 10.1023/A:1022204626604

Cite this article as:
Myerscough, N., Sylvester, P.A., Warren, B.F. et al. Glycoconj J (2001) 18: 907. doi:10.1023/A:1022204626604

Abstract

Anti-mucin variable number tandem repeat (VNTR) antibodies have been used previously to demonstrate the de novo presence of MUC5AC and MUC6 mucin in colorectal adenomas and increased synthesis of MUC2, the major secreted mucin in normal colorectal mucosa. Here we examined secreted mucins in tubular, tubulovillous and villous adenomas of the rectum using non-VNTR antibodies designed to assess mature mucin. Mucin gene messenger RNAs were detected by in situ hybridization. The anti-MUC2 non-VNTR antibody in the goblet cells of adenomas revealed a staining pattern of increased cytoplasmic, Golgi and membrane staining with no change in goblet vesicle reactivity compared with normal controls. In addition, blank goblet cell vesicle immunostaining for MUC2 was found in the transitional mucosa adjacent to all types of adenoma. Although a trend to overexpression of MUC2 was observed with in situ hybridization this was not detected with immunohistology. De novo synthesis of MUC5AC, but not MUC5B or MUC6 mucin was seen in all adenomas and transitional mucosa using immunohistochemistry. There was no correlation of MUC2 or MUC5AC mucin with polyp size or the grade of dysplasia using the non-VNTR antibodies.

This study demonstrates that anti-mucin non-VNTR antibodies reveal a different subcellular-localization in rectal adenomas compared with normal colorectal mucosa. Further, this pattern is in contrast to that reported for anti-mucin VNTR antibodies. Combined use of these reagents may benefit future assessment of these cancers.

mucin MUC2 MUC5AC immunohistochemistry in situ hybridization tubular tubulovillous and villous adenomas variable number tandem repeat 

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Neil Myerscough
    • 1
  • Paul A. Sylvester
    • 1
  • Bryan F. Warren
    • 2
  • Simon Biddolph
    • 2
  • Paul Durdey
    • 1
  • Michael G. Thomas
    • 1
  • Ingemar Carlstedt
    • 3
  • Anthony P. Corfield
    • 1
  1. 1.University Department of MedicineBristol Royal InfirmaryBristolUK
  2. 2.Department of Cellular PathologyJohn Radcliffe Hospital, HeadingtonOxfordUK
  3. 3.Department of Cell and Molecular Biology, Level C13, BMCLund UniversityLundSweden

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