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Neurochemical Research

, Volume 22, Issue 10, pp 1187–1192 | Cite as

Ethanol-Induced Cell Death by Lipid Peroxidation in PC12 Cells

  • Albert Y. Sun
  • Yong-mei Chen
  • Marilyn James-Kracke
  • Patricia Wixom
  • Yu Cheng
Article

Abstract

Free radical generation is hypothesized to be the cause of alcohol-induced tissue injury. Using fluorescent cis-parinaric acid and TBARS, lipid peroxidation was shown to be increased in the presence of trace amounts of free ferrous ion in PC12 cells. This increase in lipid peroxidation was enhanced by ethanol in a dose dependent manner and also correlated with loss of cell viability, as measured by increased release of lactate dehydrogenase (LDH). Resveratrol, a potent antioxidant, had a protective effect against lipid peroxidation and cell death. These findings strongly suggest that ethanol-induced tissue injury and cell death is a free radical mediated process, and may be important in alcohol-related premature aging and other degenerative diseases.

Lipid Peroxidation ethanol Cis-Parinaric acid = fluorescent probe for lipid peroxidation Resveratrol = antioxidant from grapes 

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Copyright information

© Plenum Publishing Corporation 1997

Authors and Affiliations

  • Albert Y. Sun
    • 1
  • Yong-mei Chen
    • 1
  • Marilyn James-Kracke
    • 1
  • Patricia Wixom
    • 1
  • Yu Cheng
    • 2
  1. 1.Department of PharmacologyUniversity of MissouriColumbia
  2. 2.Department of NeurologyWashington UniversitySt. Louis

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