Biotechnology Letters

, Volume 25, Issue 2, pp 143–147 | Cite as

Arrest of cell cycle by inhibition of ribonucleotide reductase induces accumulation of NAD+ by Mn2+-supplemented growth of Corynebacterium ammoniagenes

  • Bouziane Abbouni
  • Hesham M. Elhariry
  • Georg AulingEmail author


Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 μM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron microscopy (SEM) showed a restricted elongation characteristic of arrest of the cell cycle in coryneform bacteria. The cultures treated with HU or MP had, respectively, a fourfold or sixfold enhanced accumulation of NAD+ by a salvage biosynthetic pathway. An assay of nucleotide-permeable cells for ribonucleotide reductase activity using [3H-CDP] as substrate revealed a pre-early and complete decline of DNA precursor biosynthesis not found in the untreated control. Overproduction of NAD+ is an alternative to the conventional fermentation process using Mn2+ deficiency. A simple model is presented to discuss the metabolic regulation of the new process based on the presence of a manganese ribonucleotide reductase (Mn-RNR) in the producing strain.

cell cycle,Corynebacterium ammoniagenes elongation hydroxyurea manganese-ribonucleotide reductase NAD+ p-methoxyphenol scanning electron microscopy 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Bouziane Abbouni
    • 1
  • Hesham M. Elhariry
    • 1
  • Georg Auling
    • 1
    Email author
  1. 1.Institut für MikrobiologieUniversität HannoverHannoverGermany

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