Antiinflammatory screening of the medicinal plant Gynura procumbens
- 753 Downloads
Gynura procumbens isused in Thai folk medicine to treat topicalinflammation, rheumatism, and viral ailments. In thepresent work, attempts were made to verify the folkmedicinal claim that the crude ethanolic extract ofG. procumbens has antinflammatory action and torelate the activity to particular fractions using acroton oil-induced mouse ear inflammation model. Theoriginal ethanolic extract of G. procumbens waspartitioned between water and ethyl acetate. Theresidues were subjected to antinflammatoryevaluation. While the water extract did not show anyantinflammatory activity, the administration of theoriginal organic extract significantly inhibited theincrease in ear thickness in response to crotonoil (n = 5). The activity of 0.75 mg/ear originalorganic extract showed similar antinflammatoryactivity (inhibition 65.2%) to that of 6 mg/earhydrocortisone 21-hemisuccinate sodium salt(inhibition 64.8%). The organic extract was thenfractionated with a series of solvents in order ofincreasing polarity. Each fraction was dried,dissolved in acetone and monitored using the samebioassay. These experiments showed that the hexaneand toluene fractions showed significant inhibitionsof 44.6% and 34.8%, respectively. These twofractions had similar activities to 4 mg/ear ofhydrocortisone (inhibition 35.0%). The possiblechemical constituents in the extracts and fractionswere investigated using thin layer chromatography andspecific color reagents. These tests showed thatsteroids might be one class of antinflammatorycompounds in this plant.
Unable to display preview. Download preview PDF.
- 1.Perry LM, Metzger J (1980) Medicinal Plants of East and South East Asia: Attributed Properties and Uses. Mit Press, Cambridge. pp 94–95.Google Scholar
- 2.Roeder E, Eckert, Wiedenfeld H (1996) Pyrrolizidine Alkaloids from Gynura divaricata. Planta Med 62: 386.Google Scholar
- 3.Sadikun A, Aminah I, Ismail N, Ibrabim P (1996) Sterols and sterol glycosides from the leaves of Gynura procumbens. Natural Product Sciences 2(1): 19–23.Google Scholar
- 4.Davie AS, Wilson JC, Kiefel MJ, Raudies E, Jariksem S, Jiratchariyakul W, Iskander MN. Isolation and characterisation of several components from the aerial parts of Gynura procumbens. (unpublished)Google Scholar
- 5.Cannell RJP (1998) How to approach the isolation of a natural product. In Cannell RJP (ed), Natural Product Isolation. Totowa: Humana Press, pp 1–51.Google Scholar
- 6.Williamson EM, Okpako DT, Evans FJ (1996) Selection, Preparation and Pharmacological Evaluation of Plant Material. Chichester: John Wiley & Sons.Google Scholar
- 7.Wagner H, Bladt S (1996) Plant Drug Analysis, A Thin Layer Chromatography Atlas. New York: Springer-Verlag Berlin Heidelberg.Google Scholar
- 8.Heiman AS, Kim HP, Taraporewala IB, Lee HJ (1989) A novel class of local antiinflammatory steroids. Arzneim-Forsch/Drug Res 39(1): 262–267.Google Scholar
- 9.Kwon T, Heiman AS, Oriaku ET, Yoon K, Lee HJ (1995) New steroidal antiinflammatory antedrugs, steroidal [16?,17?-d]-3'-carbethoxyisoxazo lines. J Med Chem 38: 1048–1051.Google Scholar
- 10.Martindale, The Extra Pharmacopoeia, 32th edition. Parfitt K (ed), (1999). London: Pharmaceutical Press, pp 1043–1045.Google Scholar