Cellular and Molecular Neurobiology

, Volume 22, Issue 5–6, pp 783–795 | Cite as

Role of Endothelin Type B Receptor in NO/cGMP Signaling Pathway in Rat Median Eminence

  • Yaira Mathison
  • Anita Israel


We studied the effect of endothelins (ETs) on receptor-mediated NO/cGMP signaling in rat arcuate nucleus–median eminence (AN-ME) fragments, an hypothalamic structure known to contain a rich plexus of nitric oxide synthase (NOS)-containing neurons and fibers together with densely arranged ETB-receptor-like immunoreactive fibers. NOS activity was determined measuring the conversion of [3H] arginine to [3H] citrulline, as an index of NO produced. cGMP production was determined by radio immunoassay. ET-1, ET-3, and the selective ETB receptor agonist, IRL1620, significantly increased cGMP formation and NOS activity. Preincubation of AN-ME fragment with L-arginine analog, N-nitro-L-arginine (L-NAME), inhibited ET-1 or IRL1620-stimulated cGMP formation. The addition of the selective ETB receptor antagonist, BQ788, blocked ET-1-, ET-3-, or IRL1620-induced increase in NOS activity and cGMP generation, while BQ123, a selective ETA receptor antagonist, was ineffective. Our results demonstrate that in whole rat AN-ME fragments, ETs stimulate NO/cGMP signaling pathway through the interaction with the ETB receptor subtype, supporting the concept that ETs may represent an important regulator of reproductive and neuroendocrine function.

nitric oxide cGMP endothelin ETB receptor median eminence 


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Copyright information

© Plenum Publishing Corporation 2002

Authors and Affiliations

  • Yaira Mathison
    • 1
  • Anita Israel
    • 2
  1. 1.School of Medicine José María VargasUniversidad Central de VenezuelaCaracasVenezuela
  2. 2.School of Pharmacy, Laboratory of NeuropeptidesUniversidad Central deVenezuela, Caracas, Venezuela;

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