Molecular and Cellular Biochemistry

, Volume 243, Issue 1–2, pp 153–160 | Cite as

Quercetin suppresses proinflammatory cytokines production through MAP kinases and NF-κB pathway in lipopolysaccharide-stimulated macrophage

  • Sung-Yeon Cho
  • Sang-Joon Park
  • Myung-Ja Kwon
  • Tae-Sook Jeong
  • Song-Hae Bok
  • Woo-Young Choi
  • Won-Il Jeong
  • Si-Yun Ryu
  • Sun-Hee Do
  • Cha-Soo Lee
  • Jae-Chan Song
  • Kyu-Shik Jeong
Article

Abstract

Quercetin is a flavonoid molecule ubiquitous in nature and functions as an anti-oxidant and anti-inflammatory agent with little toxicity in vivo and in vitro. Dose- and time-dependent effect of quercetin has been investigated on proinflammatory cytokine expression and NO production, focusing on its effects on the MAP kinases and the NF-κB signal transduction pathways in LPS-stimulated RAW 264.7 cells by using RT-PCR and immunoblotting. Quercetin strongly reduced activation of phosphorylated ERK kinase and p38 MAP kinase but not JNK MAP kinase by LPS treatment. In addition, quercetin treatment inhibited NF-κB activation through stabilization of the NF-κB/IκB complex and IκB degradation and proinflammatory cytokines and NO/iNOS expression. Quercetin may exert its anti-inflammatory and immunomodulatory properties in the effect molecules such as proinflammatory cytokines and NO/iNOS by suppressing the activation of ERK and p38 MAP kinase, and NF-κB/IκB signal transduction pathways.

quercetin LPS anti-inflammation MAP-kinases NF-κB 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Sung-Yeon Cho
    • 1
  • Sang-Joon Park
    • 1
  • Myung-Ja Kwon
    • 1
  • Tae-Sook Jeong
    • 1
  • Song-Hae Bok
    • 1
  • Woo-Young Choi
    • 2
  • Won-Il Jeong
    • 3
  • Si-Yun Ryu
    • 4
  • Sun-Hee Do
    • 3
  • Cha-Soo Lee
    • 3
  • Jae-Chan Song
    • 3
  • Kyu-Shik Jeong
    • 3
  1. 1.Korea Research Institute of Bioscience and BiotechnologyYusung, Taejon CitySouth Korea
  2. 2.College of AgricultureChungnam National UniversityYusung, Taejon CitySouth Korea
  3. 3.College of Veterinary MedicineKyungpook National UniversityDaegu CitySouth Korea
  4. 4.College of Veterinary MedicineChungnam National UniversityYusung, Taejon CitySouth Korea

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