, Volume 129, Issue 1, pp 15–23 | Cite as

Genetic relationships among annual and perennial wild species of Cicer using inter simple sequence repeat (ISSR) polymorphism

  • P.N. Rajesh
  • V.J. Sant
  • V.S. Gupta
  • F.J. Muehlbauer
  • P.K. Ranjekar


Wild Cicer germplasm is known to havegenes for disease resistance, stresstolerance and other important traits, andhence could be exploited for improvingcultivated genotypes. However, only fewCicer species are interfertile and itis essential to overcome crossabilitybarriers to utilize the germplasm moreeffectively. Genetic diversity analysis ofCicer species can give importantclues in understanding speciesrelationships and may assist in developingand planning breeding strategies. Weselected 6 annual and 7 perennial wildspecies, which were amplified using 15 ISSRprimers and UPGMA and AMOVA were used toevaluate the genetic diversity. On anaverage, 6.6 polymorphic bands per primerwere observed. Cluster analysis using theUPGMA algorithm indicated three majorgroups of species at the similarity valueof 0.60 with many subclusters. Theclustering pattern was in agreement withthe data based on crossability, seedstorage protein, isozyme, allozyme and RAPDmarker analysis. The among-populationcomponent of annual and perennial groupscalculated using AMOVA accounted for39.00%. Our results suggested that wildannuals of Cicer were notmonophyletic in nature.

Cicer sections crossability genetic diversity species 


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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • P.N. Rajesh
    • 1
  • V.J. Sant
    • 1
  • V.S. Gupta
    • 1
  • F.J. Muehlbauer
    • 2
  • P.K. Ranjekar
    • 1
  1. 1.Division of Biochemical SciencesNational Chemical Laboratory, PuneMaharashtra StateIndia
  2. 2.USDA-ARS, Grain Legume Genetics and Physiology Unit and Department of Crop and Soil SciencesWashington State UniversityPullmanU.S.A

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