Euphytica

, Volume 129, Issue 1, pp 33–41

Identification of olive-tree cultivars with SCAR markers

  • Rocío Bautista
  • Remedios Crespillo
  • Francisco M. Cánovas
  • M. Gonzalo Claros
Article

Abstract

There is an urgent need for the developmentof early identification techniques inolive-trees due to the economic importanceof cultivar identification in periods ofexpansion like now. We have been able toidentify 22 olive-tree cultivars using only10 different, specific, repeatable markers.These markers were designed by the cloningof significant RAPD bands obtained in PCRperformed on bulked DNA to retain thegenetic variability of each cultivar.Clones were partially or totally sequencedand new primers derived from thesesequences were used to obtain SequenceCharacterised Amplified Region (SCAR)fragments. We have demonstrated that theuse of the 10 SCAR markers is enough toprovide a simple, cheap, and reliableprocedure to identify 22 geographicallyrelated olive-tree cultivars.

molecular markers Olea europaea olive tree SCAR 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Rocío Bautista
    • 1
  • Remedios Crespillo
    • 1
  • Francisco M. Cánovas
    • 1
  • M. Gonzalo Claros
    • 1
  1. 1.Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias e Instituto Andaluz de Biotecnología, Unidad Asociada UMA-CSICUniversidad de MálagaMálagaSpain

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