Chromosome Research

, Volume 10, Issue 8, pp 669–683

The phylogeny of howler monkeys (Alouatta, Platyrrhini): Reconstruction by multicolor cross-species chromosome painting

  • Edivaldo H. C. de Oliveira
  • Michaela Neusser
  • Wilsea B. Figueiredo
  • Cleusa Nagamachi
  • Julio Cesar Pieczarka
  • Ives J. Sbalqueiro
  • Johannes Wienberg
  • Stefan Müller
Article

Abstract

We performed multidirectional chromosome painting in a comparative cytogenetic study of the three howler monkey species Alouatta fusca, A. caraya and A. seniculus macconnelli (Atelinae, Platyrrhini) in order to reconstruct phylogenetic relationships within this genus. Comparative genome maps between these species were established by multicolor fluorescence in-situ hybridization (FISH) employing human, Saguinus oedipus and Lagothrix lagothricha chromosome-specific probes. The three species included in this study and previously analyzed howler monkey species were subjected to a phylogenetic analysis on the basis of a data matrix comprised of 98 discrete molecular cytogenetic characters. The results revealed that howler monkeys represent the genus with the most extensive karyotype diversity within Platyrrhini so far analyzed with high levels of intraspecific chromosomal variability. Two different multiple sex chromosome systems were identified. The phylogenetic analysis indicated that Alouatta is a monophyletic clade which can be derived from a proposed ancestral Atelinae karyotype of 2n=62 chromosomes by a chromosome fusion, a fission, a Y-autosomal translocation and a pericentric inversion. Following these suggestions, the genus Alouatta can be divided into two distinct species groups: the first includes A. caraya and A. belzebul, the second A. s. macconnelli, A. sara, A. s. arctoidea and A. fusca.

Alouatta chromosomal phylogeny comparative molecular cytogenetics fluorescence in-situ hybridization Primates 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Edivaldo H. C. de Oliveira
    • 1
    • 2
  • Michaela Neusser
    • 1
  • Wilsea B. Figueiredo
    • 3
  • Cleusa Nagamachi
    • 2
  • Julio Cesar Pieczarka
    • 2
  • Ives J. Sbalqueiro
    • 2
  • Johannes Wienberg
    • 1
    • 3
  • Stefan Müller
    • 1
  1. 1.Department Biologie II, HumangenetikLudwig-Maximilians-Universität, Richard-Wagner-Strasse 10MunichGermany; Tel
  2. 2.Laboratório de Citogenética, Depto de GenéticaUniversidade Federal do ParáBelém, PABrazil
  3. 3.Campus de SantarémUniversidade Federal do ParáSantarém, PABrazil

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