Environmental Monitoring and Assessment

, Volume 81, Issue 1–3, pp 257–268

Interlaboratory Variability of Amphipod Sediment Toxicity Tests in a Cooperative Regional Monitoring Program

  • Steven M. Bay
  • Andrew Jirik
  • Stanford Asato


Marine sediment toxicity tests are widely applied in monitoring programs, yet relatively little is known about the comparability of data from different laboratories. The need for comparability information is increased in cooperative monitoring programs, where multiple laboratories (often with variable skill levels) perform toxicity tests. An interlaboratory comparison exercise was conducted among seven laboratories in order to document the comparability of sediment toxicity measurements during the Bight'98 regional sediment survey in southern California. Sediments from four stations in Los Angeles and Long Beach Harbors were tested using a 10-day survival test of the amphipod Eohaustorius estuarius. All laboratories successfully performed the sediment test and associated reference toxicant test. Statistically significant differences were found in mean amphipod survival rates among some laboratories for the field-collected sediments, but there was little evidence of a consistent bias among laboratories. Although the reference toxicant test indicated a five-fold variation in test sensitivity among laboratories, these results were not accurate predictors of interlaboratory performance for the sediment tests. The laboratories demonstrated excellent concordance (Kendall's W = 0.91) in ranking the field-collected sediments by toxicity. Agreement on classifying the sediments into categories (nontoxic, moderately toxic, and highly toxic) based upon the percent of survival was best for highly toxic sediments. An analysis of test precision based upon the variance among replicates within a test indicated that the measured survival rate for a sample may vary by up to 12 percentage points from the actual response.

sediment toxicity southern California variability amphipod regional monitoring 


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  1. ASTM (American Society for Testing and Materials): 1992, Guide for Conducting 10–day Static Sediment Toxicity Tests with Marine and Estuarine Amphipods. E1367–92. ASTM 1992 Annual Book of Standards, Vol. 11.04, Philadelphia, PA.Google Scholar
  2. Anderson, B. S., Hunt, J. W., Tudor, S., Newman, J., Tjeerdema, R. S., Fairey, R. S., Oakden, J, Bretz, C., Wilson, C. J., LaCaro, F., Stephenson, M. D., Puckett, H. M., Long, E. R., Fleming, T., Summers, K.: 1997, Chemistry, Toxicity, and Benthic Community Conditions in Selected Sediments of the Southern California Bays and Estuaries. Final Report, State Water Resources Control Board, Sacramento, CA, 157 pp. + app.Google Scholar
  3. Bay, S. M.: 1992, ‘Temporal and Spatial Changes in Sediment Toxicity in Santa Monica Bay’, in: Cross J. (ed), Annual Report 1990–91 and 1991–92, Southern California Coastal Water Research Project, Westminster, CA, pp. 81–87.Google Scholar
  4. Bay, S. M., Greenstein, D. J., Jirik, A. W., Brown, J.S.: 1998, Southern California Bight 1994 Pilot Project: VI. Sediment Toxicity. Southern California Coastal Water Research Project, Westminster, CA, 65 pp.Google Scholar
  5. Bight'98 Steering Committee: 1998, Southern California Bight 1998 regional monitoring survey (Bight'98): Coastal Ecology Workplan. Southern California Coastal Water Research Project, Westminster, CA, 66 pp.Google Scholar
  6. Fairey, R. S., Bretz, C., Lamerdin, S., Hunt, J. W., Anderson, B. S., Tudor, S., Wilson, C. J., LaCaro, F., Stephenson, M. D., Puckett, H. M., Long, E. R.: 1996, Chemistry, Toxicity, and Benthic Community Conditions of Selected Sediments of the San Diego Bay Region. Final Report, State Water Resources Control Board, Sacramento, CA, 179 pp. + app.Google Scholar
  7. Long, E. R.: 2000, ‘Degraded Sediment Quality in U.S. Estuaries: A Review of Magnitude and Ecological Implications’, Ecological Applications 10, 338–349.Google Scholar
  8. Long, E. R., Field, L. J., MacDonald, D. D.: 1998, ‘Predicting Toxicity in Marine Sediments with Numerical Sediment Quality Guidelines’, Environmental Toxicology and Chemistry 17, 714–727.Google Scholar
  9. Long, E. R., MacDonald, D., Smith, S., Calder, F.: 1995, ‘Incidence of Adverse Biological Effects within Ranges of Chemical Concentrations in Marine and Estuarine Sediments’, Environmental Management 19, 81–97.Google Scholar
  10. Mearns, A. J., Swartz, R. C., Cummins, J. M., Dinnel, P. A., Plesha, P., Chapman, P. M.: 1986, ‘Interlaboratory Comparison of a Sediment Toxicity Test using the Marine Amphipod Rhepoxynius abronius’, Marine Environmental Research 19, 13–37.Google Scholar
  11. Schlekat, C. E., Scott, K. J., Swartz, R. C., Albrecht, B., Antrim, L., Doe, K., Douglas, S., Ferretti, J. A., Hansen, D. J., Moore, D. W., Mueller, C., Tang, A.: 1995, ‘Interlaboratory Comparison of a 10–day Sediment Toxicity Test Method using Ampelisca abdita, Eohaustorius estuarius, and Leptocheirus plumulosus’, Environmental Toxicology and Chemistry 14, 2163–2174.Google Scholar
  12. Swartz, R.C., Cole, F.A., Schultz, D.W., DeBen, W.A.: 1986, ‘Ecological Changes in the Southern California Bight near a Large Sewage Outfall: Benthic Conditions in 1980 and 1983’, Marine Ecology Progress Series 31, 1–13.Google Scholar
  13. USEPA (U.S. Environmental Protection Agency): 1994a, Methods for Assessing the Toxicity of Sediment-associated Contaminants with Estuarine and Marine Amphipods. EPA/600/R-94/025. U.S. Environmental Protection Agency, Washington, DC.Google Scholar
  14. USEPA (U.S. Environmental Protection Agency): 1994b, Methods for Measuring the Toxicity and Bioaccumulation of Sediment-Associated Contaminants with Freshwater Invertebrates. EPA/600/R-94/024. U.S. Environmental Protection Agency, Duluth, MN.Google Scholar
  15. USEPA (U.S. Environmental Protection Agency): 2001, Methods for Assessing the Chronic Toxicity of Marine and Estuarine Sediment-associated Contaminants with the Amphipod Leptocheirus plumulosus. EPA/600/R-01/020. U.S. Environmental Protection Agency, Washington, DC.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Steven M. Bay
    • 1
  • Andrew Jirik
    • 2
  • Stanford Asato
    • 3
  1. 1.Southern California Coastal Water Research ProjectWestminsterU.S.A
  2. 2.Environmental Management Dept.Port of Los AngelesSan PedroU.S.A
  3. 3.Environmental Monitoring Div.City of Los AngelesPlaya del ReyU.S.A

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