European Journal of Plant Pathology

, Volume 108, Issue 9, pp 909–912 | Cite as

PCR–RFLP and Sequence Data Delineate Three Diaporthe Species Associated with Stone and Pome Fruit Trees in South Africa

  • Ntsane Moleleki
  • Oliver Preisig
  • Michael John Wingfield
  • Pedro Willem Crous
  • Brenda Diana WingfieldEmail author


Diaporthe species include canker pathogens on a wide variety of hosts. In South Africa, Diaporthe canker of apple, pear and plum rootstocks has been attributed to Diaporthe ambigua. Recently, we recognized that isolates of D. ambigua exhibited different morphological features and thus questioned the identity of these isolates. A small set of isolates was thus chosen for comparison using DNA-based methods. Polymerase chain reaction amplification of ribosomal DNA, Restriction Fragment Length Polymorphisms and DNA sequencing revealed that isolates which had been regarded as D. ambigua in the past were three distinct species. These are D. ambigua, D. perjuncta and an unknown Phomopsis sp. This discovery has special relevance to research done on a dsRNA virus previously thought to occur in D. ambigua and now shown to infect D. perjuncta.

canker pathogen dsRNA mycovirus ribosomal DNA 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Ntsane Moleleki
    • 1
  • Oliver Preisig
    • 2
  • Michael John Wingfield
    • 2
  • Pedro Willem Crous
    • 3
  • Brenda Diana Wingfield
    • 4
    Email author
  1. 1.Department of GeneticsUniversity of PretoriaPretoriaSouth Africa
  2. 2.Forestry and Agricultural Biotechnology Institute (FABI)University of PretoriaPretoriaSouth Africa
  3. 3.Centraalbureau voor SchimmelculturesUtrechtThe Netherlands
  4. 4.Department of GeneticsUniversity of PretoriaPretoriaSouth Africa

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