Journal of Biological Physics

, Volume 28, Issue 4, pp 605–617 | Cite as

Controlling and Measuring Local Composition and Properties in Lipid Bilayer Membranes

  • T.G. D'Onofrio
  • C.W. Binns
  • E.H. Muth
  • C.D. Keating
  • P.S. Weiss
Article

Abstract

Local composition, structure, morphology, and phase are interrelated in lipid bilayer membranes. This gives us the opportunity to control one or more of these properties by manipulating others. We investigate theserelationships with combinations of simultaneous two-color widefield fluorescence imaging, three-dimensional rendering of vesicle domains, andmanipulation of the vesicle morphology via optical trapping and micropipetteaspiration. We describe methods to modulate, to measure, and to probe thelocal structure of model membranes through control of membrane curvature inliposomes.

fluorescence imaging phase domains vesicle curvature 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • T.G. D'Onofrio
    • 1
  • C.W. Binns
    • 1
  • E.H. Muth
    • 1
  • C.D. Keating
    • 1
  • P.S. Weiss
    • 1
  1. 1.Department of ChemistryThe Pennsylvania State UniversityUniversity ParkUSA

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