Plant Growth Regulation

, Volume 38, Issue 2, pp 141–143

The levels of GA3 and GA20 may be associated with dormancy release in Onopordum nervosum seeds

  • C. Pérez
  • F. Pérez-García
  • H. Fernández
  • M.A. Revilla
Article

DOI: 10.1023/A:1021210217659

Cite this article as:
Pérez, C., Pérez-García, F., Fernández, H. et al. Plant Growth Regulation (2002) 38: 141. doi:10.1023/A:1021210217659

Abstract

Endogenous levels of two gibberellins, GA3 andGA20, were quantified in unimbibed Onopordumnervosum seeds collected from two different populations, whichshoweddifferences in their germination capacity. After purifying the seed extracts,gibberellin levels were evaluated by gas chromatography mass spectrometry byusing selected ion monitoring (GC-MS-SIM) adding deuterated gibberellins asinternal standards. The intraspecific differences in germination capacity wereassociated with differences in the endogenous levels of both gibberellins. Thecontents of GA3 and GA20 in seeds with high germinationrate were twice and five times higher, respectively, than those from seeds witha low germination rate, indicating a possible role of gibberellins in dormancyrelease in this plant species.

Dormancy GC-MS-SIM Germination Gibberellin Onopordum nervosum Seed 

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • C. Pérez
    • 1
  • F. Pérez-García
    • 1
  • H. Fernández
    • 2
  • M.A. Revilla
    • 2
  1. 1.Dpt. Biología Vegetal, ETSIA, UPMCiudad UniversitariaMadridSpain
  2. 2.Dpt. B.O.S. c)Catedrático R. Uría s/n, Lab. Fisiología VegetalOviedoSpain

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