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Micropropagation of Venus fly trap by shoot culture

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Abstract

In order to establish and optimize an in vitro micropropagation protocol of Venus fly trap (Dionaea muscipula Ellis), a carnivorous plant, the effects of medium type, MS medium concentration, pH, and cytokinin and auxin types on shoot proliferation and root formation were investigated using 3-month-old shoots. The shoot proliferation was most effective in 2.3 μM kinetin-supplemented 1/3MS medium at pH 5.5. The best conditions for rooting were 1/3MS medium supplemented with 0.5 μM IBA. All subcultured shoots produced extensive root systems after 5–6 weeks culture. When plantlets after rooting were planted in plastic pots filled with 1:1 peat moss and sand, the survival rate of plantlets was almost 100%, exhibiting normal development. With subculture every 8 weeks, hundreds of the plants were propagated from a single plant within a year.

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Authors and Affiliations

  1. Institute of Agricultural Science and Technology, South Korea

    Gi-Won Jang, Kwang-Soo Kim & Ro-Dong Park

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  1. Gi-Won Jang
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  2. Kwang-Soo Kim
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  3. Ro-Dong Park
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Correspondence to Gi-Won Jang.

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Jang, GW., Kim, KS. & Park, RD. Micropropagation of Venus fly trap by shoot culture. Plant Cell, Tissue and Organ Culture 72, 95–98 (2003). https://doi.org/10.1023/A:1021203811457

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  • DOI: https://doi.org/10.1023/A:1021203811457

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