, Volume 477, Issue 1–3, pp 1–13 | Cite as

Trichodesmium in coastal waters of Tanzania: diversity, seasonality, nitrogen and carbon fixation

  • Charles Lugomela
  • Thomas J. Lyimo
  • Ian Bryceson
  • Adelaida K. Semesi
  • Birgitta Bergman


Seasonal distribution, nitrogen fixation and primary productivity of Trichodesmium species were intermittently studied in the coastal waters of Tanzania. Samples were collected in 1975/6, 1980, 1993/4, 1994/5 and 1998/9. Four colony forming species were found, i.e. T. erythraeum, T. tenue, T. thiebautii and one unidentified Trichodesmium sp. while T. contortum was rarely encountered, and only as individual trichomes. T. erythraeum was most abundant, although other Trichodesmiumspecies dominated during particular periods of the year. The occurrence of Trichodesmium showed a consistent seasonal pattern. High Trichodesmium biomass was promoted by the NE monsoon (December–April) while it was low or absent during the SE monsoon (June–October). The biomass was highest at the surface especially during calm weather periods. The NE monsoon was characterized by elevated rainfall, temperature and nitrate concentrations while salinity, light intensity and turbidity tended to decrease. Phosphate concentrations did not show systematic variations with season. The rate of nitrogen fixation by Trichodesmium species in surface waters was 1.8 ± 1.6 pmol N trichome−1 h−1 giving an average N2 fixation of 42.7 mmol N m−3y−1. The mean rate of carbon fixation was 1.15 ± 0.3 ng C trichomes−1 h−1 in the upper 5 m depth. It is estimated that Trichodesmium contributes about 0.03–20% of the total CO2 fixation in the coastal surface waters during the SE and NE monsoon, respectively.

Trichodesmiumspp. seasonal distribution nitrogen fixation carbon fixation western Indian Ocean 


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  1. Bergman, B., J. R. Gallon, A. N. Rai & L. J Stal, 1997. N2 fixation by non-heterocystous cyanobacteria. FEMS Microbiol. Rev. 19: 139–185.Google Scholar
  2. Berman-Frank, I., P. Lundgren, Y. Chen, H. Küpper, Z. Kolber, B. Bergman & P. Falkowski, 2001. Segregation of nitrogen fixation and oxygenic photosynthesis in the marine cyanobacterium Trichodesmium. Science 294: 1534–1537.Google Scholar
  3. Bryceson, I., 1982. Seasonality of oceanographic conditions and phytoplankton in Dar es Salaam waters. Univ. Sci. J. (Dar. Univ.) 8: 66–76.Google Scholar
  4. Bryceson, I. & P. Fay, 1981. Nitrogen fixation in Oscillatoria (Trichodesmium) erythraea in relation to bundle formation and trichome differentiation. Mar. Biol. 61: 159–166.Google Scholar
  5. Burris, R. H., 1972. Measurement of biological N2 fixation with 15N and acetylene. In Sorokin, Y. I. & H. Kadota (eds), Microbial Production and Decomposition in Freshwaters. Blackwell, Oxford: 3–14.Google Scholar
  6. Capone, D. G., 1993. Determination of nitrogenase activity in aquatic samples using the acetylene reduction procedure. In Kemp, P. F., B. F. Sherr, E. V. Sherr & J. J. Cole (eds), Handbook of Methods in Aquatic Ecology. Lewis Publishers, Tokyo: 621–631.Google Scholar
  7. Capone, D. G., J. P. Zehr, H. W. Paerl, B. Bergman & E. J. Carpenter, 1997. Trichodesmium, a globally significant marine cyanobacterium. Science 276: 1221–1229.Google Scholar
  8. Capone, D. G., A. Subramaniam, J. P. Montoya, M. Voss, C. Humborg, A. M. Johansen, R. L. Siefert & E. J. Carpenter, 1998. An extensive bloom of the N2-fixing cyanobacterium Trichodesmium erythraeum in the Arabian Sea. Mar. Ecol. Prog. Ser. 172: 281–292.Google Scholar
  9. Capone, D. G. & E. J. Carpenter, 1999. Nitrogen fixation by marine cyanobacteria: historical and global perspectives. In Charpy, L. & A. W. D. Larkum (eds), Marine Cyanobacteria. Mosée Océanographique, Monaco: 235–256.Google Scholar
  10. Carpenter, E. J. & C. C. Price, 1976. Marine Oscillatoria (Trichodesmium): an explanation for aerobic nitrogen fixation without heterocysts. Science 191: 1278–1280.Google Scholar
  11. Carpenter, E. J. & C. C. Price, 1977. Nitrogen fixation distribution and production of Oscillatoria (Trichodesmium) spp. in the western Sargasso and Caribbean Seas. Limnol. Oceanogr. 22: 60–72.Google Scholar
  12. Carpenter, E. J., M. I. Scranton, P. C. Novelli & A. Michaelis, 1987. Validity of N2 fixation rate measurements in marine Oscillatoria (Trichodesmium). J. Plankton Res. 9: 1047–1056.Google Scholar
  13. Carpenter, E. J. & K. Romans, 1991. Major role of the cyanobacterium Trichodesmium in nutrient cycling in the North Atlantic Ocean. Science 254: 1356–1358.Google Scholar
  14. Chang, J., 2000. Precision of different methods used for estimating the abundance of the nitrogen fixing marine cyanobacterium, Trichodesmium Ehrenberg. J. exp. mar. Biol. 245: 215–224.Google Scholar
  15. Devassy, V. P., P. M. A. Bhattathiri & S. Z. Qasim, 1978. Trichodesmium phenomenon. Ind. J. mar. Sci. 7: 168–186.Google Scholar
  16. Duce, R. A., S. P. Liss, J. T. Merrill, E. L. Atlas, P. Buat-Menard, B. B. Hicks, J. M. Miller, J. M. Prospero, R. Arimoto, T. M. Church, W. Ellis, J. N. Galloway, L. Hansen, T. D. Jickells, A. H. Knap, K. H. Reinhardt, B. Schneider, A. Soudine, J. J. Tokos, S. Tsunogai, R. Wollast & M. Zhou, 1991. The atmospheric input of trace species to the world ocean. Global Biogeochem. Cycles 5: 193–259.Google Scholar
  17. Gallon, J. R., D. A. Jones & T. S. Page, 1996. Trichodesmium, the paradoxical diazotroph. Algol. Stud. 83: 215–243.Google Scholar
  18. Glibert, P. M. & D. A. Bronk, 1994. Release of dissolved organic nitrogen by marine diazotrophic cyanobacteria, Trichodesmium spp. Appl. envir. Microbiol. 60: 3996–4000.Google Scholar
  19. Hawser, S. P. & G. A. Codd, 1992. Toxicity of Trichodesmium blooms from Caribbean waters. In Carpenter, E. J., D. G. Capone & J. G. Rueter (eds), Marine Pelagic Cyanobacteria: Trichodesmium and other Diazotrophs. Kluwer Academic Publishers, Dordrecht, The Netherlands: 319–329.Google Scholar
  20. Hawser, S. P., J. M. O'Neil, M. R. Romans & G. A. Codd, 1992. Toxicity of blooms of the cyanobacterium Trichodesmium to zooplankton. J. appl. Phycol. 4: 79–86.Google Scholar
  21. Hoffmann, L. 1999. Marine cyanobacteria in tropical regions: diversity and ecology. Eur. J. Phycol. 34: 371–379.Google Scholar
  22. Janson, S., P. J. A. Siddiqui, A. E. Walsby, K.M. Romans, E. J. Carpenter & B. Bergman, 1995. Cytomorphological characterization of the planktonic diazotrophic cyanobacteria Trichodesmium spp. from the Indian Ocean and Caribbean and Sargasso Seas. J. Phycol. 31: 463–477.Google Scholar
  23. Karl, D. M., R. Letelier, D. V. Hebel, D. F. Bird & C. D. Winn, 1992. Trichodesmium blooms and new nitrogen in the North Pacific gyre. In Carpenter, E. J., D. G. Capone & J. G. Rueter (eds), Marine Pelagic Cyanobacteria: Trichodesmium and other Diazotrophs. Kluwer Academic Publishers, Dordrecht, The Netherlands: 219–237.Google Scholar
  24. Karl, D., R., Letelier, L. Tupas, J. Done, J. Christian & D. Hebel, 1997. The role of nitrogen fixation in biogeochemical cycling in the subtropical North Pacific Ocean. Nature 388: 533–538.Google Scholar
  25. Kromkamp, J., M. De Bie, N. Goosen, J. Peene, P. V. Rijswijk, J. Sinkie & G. C. A. Duineveld, 1997. Primary production by phytoplankton along the Kenyan coast during the SE monsoon and November intermonsoon 1992, and the occurrence of Trichodesmium. Deep Sea Res. 44: 1195–1212.Google Scholar
  26. Lenes, J. M., B. P. Darrows, C. Cattrall, C. A. Heil, M. Callahan, G. A. Vargo & R. H. Byrne, 2001. Iron fertilization and the Trichodesmium response on the West Florida shelf. Limnol. Oceanogr. 46: 1261–1277.Google Scholar
  27. Letelier, R. M. & D.M. Karl, 1998. Trichodesmium spp. Physiology and nutrient fluxes in the North Pacific subtropical gyre. Aquat. Microb. Ecol. 15: 265–276.Google Scholar
  28. Lugomela, C., P. Wallberg & T. G. Nielsen, 2001. Plankton composition and cycling of carbon during the rainy season in a tropical coastal ecosystem, Zanzibar, Tanzania. J. Plankton Res. 23: 1121–1136.Google Scholar
  29. Lund, J. W. G., G. Kipling & E. D. Le Gren, 1958. The inverted microscope method of estimating algal numbers and the statistical basis of estimates by counting. Hydrobiologia 11: 143–170.Google Scholar
  30. Lwiza, K. M. M. & P. R. Bigendako, 1988. Kunduchi tides. Tanzania J. Sci. 14: 65–76.Google Scholar
  31. Marumo, R. & S. Nagasawa, 1976. Seasonal variation of the standing crop of a pelagic blue-green alga, Trichodesmium in the Kuroshio water. Bull. Plankton Soc. Jpn. 23: 19–25.Google Scholar
  32. McCarthy, J. J. & E. J. Carpenter, 1979. Oscillatoria (Trichodesmium) thiebautii (Cyanophyta) in the central North Atlantic Ocean. J. Phycol. 15: 75–82.Google Scholar
  33. McClanahan, T. R., 1988. Seasonality in East Africa's coastal waters. Mar. Ecol. Progr. Ser. 44: 191–199.Google Scholar
  34. Newell, B. S., 1959. The hydrography of the British East African coastal waters. Part II. Colonial Office Fishery Publication, No. 12 H.M.S.O. London: 18 pp.Google Scholar
  35. Okera, W., 1974. The zooplankton of the inshore waters of Dar es Salaam (Tanzania, S.E. Africa) with observations on the reaction to artificial light. Mar. Biol. 26: 13–25.Google Scholar
  36. O'Neil, J. M. & M. R. Romans, 1992. Grazers and associated organisms of Trichodesmium. In Carpenter, E. J., D. G. Capone & J. G. Rueter (eds), Marine Pelagic cyanobacteria: Trichodesmium and other Diazotrophs. Kluwer Academic Publishers, Dordrecht, The Netherlands: 61–73.Google Scholar
  37. O'Neil, J. M., 1999. Grazers interaction with nitrogen fixing cyanobacteria. Adaptation for N-acquisition? In Charpy, L. & A. W. D. Larkum (eds), Marine Cyanobacteria. Musée Océanographique, Monaco: 293–318.Google Scholar
  38. O'Neil, J. M., P. M. Metzler & P. M. Glibert, 1996. Ingestion of 15N2-labeled Trichodesmium spp. and ammonium regeneration by the harpacticoid copepod Macrosetella gracilis. Mar. Biol. 125: 89–96.Google Scholar
  39. Orcutt, K. M., 1999. Environmental factors regulating N2 fixation by Trichodesmium spp. in the Sargasso Sea. Ph.D. thesis, University of Wales, Swansea: 158 pp.Google Scholar
  40. Owen, N. J. P., J. N. Galloway & R. A. Duce, 1992. Episodic atmospheric nitrogen deposition to oligotrophic oceans. Nature 357: 397–399.Google Scholar
  41. Parsons, T. R., Y. Maita & C.M. Lalli, 1984. A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press, 173 pp.Google Scholar
  42. Paerl, H. W., L. E. Prufet-Bebout & C. Guo, 1994. Iron-stimulated N2 fixation and growth in natural and cultured populations of the planktonic marine cyanobacteria Trichodesmium spp. Appl. envir. Microbiol. 60: 1044–1047.Google Scholar
  43. Rhyther, J. H., J. R. Hall, A. K. Bakun & M. M. Jones, 1966. Primary production in relation to chemistry and hydrography of the western Indian Ocean. Limnol. Oceanogr. 11: 371–380.Google Scholar
  44. Romans, K. M., E. J. Carpenter & B. Bergman, 1994. Buoyancy regulation in the colonial diazotrophic cyanobacterium Trichodesmium tenue: ultrastracture and storage of carbohydrate, polyphosphate, and nitrogen. J. Phycol. 30: 935–942.Google Scholar
  45. Rueter, J. G., 1988. Iron stimulation of photosynthesis and nitrogen fixation in Anabaena 7120 and Trichodesmium (Cyanophyceae). J. Phycol. 24: 249–254.Google Scholar
  46. Rueter, J. G., K. Ohki & Y. Fujita, 1990. The effect of iron nutrition on photosynthesis and nitrogen fixation in cultures of Trichodesmium (Cyanophyceae). J. Phycol. 26: 30–35.Google Scholar
  47. Rueter, J. G., D. A. Hutchins, R. W. Smith & N. L. Unsworth, 1992. Iron nutrition of Trichodesmium. In Carpenter, E. J., D. G. Capone & J. G. Rueter (eds), Marine Pelagic Cyanobacteria: Trichodesmium and other Diazotrophs. Kluwer Academic Publishers, Dordrecht, The Netherlands: 219–237.Google Scholar
  48. Siddiqui, P. J. A., B. Bergman & E. J. Carpenter, 1992. Filamentous cyanobacteria associates of marine planktonic cyanobacterium, Trichodesmium. Phycologia 31: 326–337.Google Scholar
  49. Steven, D. M. & R. Glombitza, 1972. Oscillatory variation of a phytoplankton population in a tropical ocean. Nature 237: 105–107.Google Scholar
  50. Strickland, J. D. H. & T. R. Parsons, 1968. A Practical Handbook of Seawater Analysis. Bulletin No. 167, Fisheries Research Board of Canada, Ottawa: 311 pp.Google Scholar
  51. Utermöhl, H., 1931. Nue Wege in der quantitaiven Erfassung des Planktos. Verh. int. Ver. Limnol.: 5 pp.Google Scholar
  52. Villareal, T. A. & E. J. Carpenter, 1990. Diel buoyancy regulation in the marine diazotrophic cyanobacterium Trichodesmium thiebautii. Limnol. Oceanogr. 35: 1832–1837.Google Scholar
  53. Wallberg, P., P. R. Jonsson & R. Johnstone, 1999. Abundance, biomass and growth rates of pelagic micro-organisms in a tropical coastal ecosystem. Aquat. Microb. Ecol. 18: 175–185.Google Scholar
  54. Walsby, A. E., 1978. The properties and buoyancy providing role of gas vacuole in Trichodesmium Ehrenberg. Br. Phycol. J. 13: 103–116.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Charles Lugomela
    • 1
    • 2
  • Thomas J. Lyimo
    • 3
  • Ian Bryceson
    • 4
  • Adelaida K. Semesi
    • 3
  • Birgitta Bergman
    • 2
  1. 1.Department of Zoology and Marine BiologyUniversity of Dar es SalaamDar es SalaamTanzania
  2. 2.Department of BotanyStockholm UniversityStockholmSweden
  3. 3.Department of BotanyUniversity of Dar es SalaamDar es SalaamTanzania
  4. 4.Center for International Environment and Development Studies, NoragricAgricultural University of NorwayAasNorway

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