Journal of Chemical Ecology

, Volume 28, Issue 11, pp 2371–2376 | Cite as

Odorant Binding Protein Homologues of the Malaria Mosquito Anopheles gambiae; Possible Orthologues of the OS-E and OS-F OBPs of Drosophila melanogaster

  • Richard G. Vogt


Twenty-nine Anopheles gambiae candidate Odorant Binding Proteins (OBPs) were characterized for similarity to OBPs of Drosophila melanogaster and other insects. Twenty-five of these sequences were identified by BLAST searching the A. gambiae genome database. Several A. gambiae sequences were significantly similar to the D. melanogaster OBPs OS-E/OS-F, LUSH and PBPRP2/PBPRP5. Exon boundary comparisons suggests that two A. gambiae genes are orthologues of OS-E and OS-F, justifying the names AgamOS-E (EAA01090, AF437886) and AgamOS-F (EAA14641, AF437884). If these are orthologues, then the gene duplication establishing the OS-E and OS-F lineages predated the divergence of mosquitoes and flies. The identification of orthologous OBPs and other chemosensory genes between D. melanogaster and A. gambiae should accelerate the transfer of physiological and behavioral information between these two species.

Odorant Binding Protein OBP evolution odor detection pheromone detection chemosensory proteins Anopheles gambiae mosquito olfaction 


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  1. Biessmann, H., Walter, M. F., Dimitratos, S., and Woods, D. 2002. Isolation of cDNA clones encoding putative odourant binding proteins from the antennae of the malaria-transmitting mosquito. Anopheles gambiae. Insect Mol. Biol. 11:123–132.Google Scholar
  2. De Bruyne, M., Foster, K., and Carlson, J. R. 2001. Odor coding in the Drosophila antenna. Neuron 30:531–552.Google Scholar
  3. Galindo, K. and Smith, D. P. 2001. A large family of divergent Drosophila odorant-binding proteins expressed in gustatory and olfactory sensilla. Genetics 159: 1059–1072.Google Scholar
  4. Graham, L. A. and Davies, P. L. 2002. The odorant-binding proteins of Drosophila melanogaster: annotation and characterization of a divergent gene family. Gene 292:43–55.Google Scholar
  5. Hekmat-Scafe, D. S., Steinbrecht, R. A., and Carlson, J. R. 1997. Coexpression of two odorant binding protein homologs in Drosophila: implications for olfactory coding. J Neurosci. 17:1616–1624.Google Scholar
  6. Hekmat-Scafe, D. S., Dorit, R. L., and Carlson, J. R. 2000. Molecular evolution of odorant-binding protein genes OS-E and OS-F in Drosophila. Genetics 155:117–127.Google Scholar
  7. Ishida, Y., Cornel, A. J., and Leal, W. S. 2002. Identification and cloning of a female antenna-specific odorant-binding protein in the mosquito Culex quinquefasciatus. J. Chem. Ecol. 28:867–871.Google Scholar
  8. Robertson, H. M., Martos, R., Sears, C. R., Todres, E. Z., Walden, K. K., and Nardi, J. B. 1999. Diversity of odourant binding proteins revealed by an expressed sequence tag project on male Manduca sexta moth antennae. Insect Mol. Biol. 8:501–518.Google Scholar
  9. Shanbhag, S. R., Hekmat-Scafe, D., Kim, M. S., Park, S. K., Carlson, J. R., Pikielny, C., Smith, D. P., and Steinbrecht, R.A. 2001. Expression mosaic of odorant-binding proteins in Drosophila olfactory organs. Microsc. Res. Tech. 55:297–306.Google Scholar
  10. Vogt, R. G., Callahan, F. E., Rogers, M. E., and Dickens, J. C. 1999. Odorant Binding Protein Diversity and Distribution among the Insect Orders, as indicated by LAP, an OBP-related protein of the True Bug Lygus lineolaris (Hemiptera, Heteroptera). Chemical Senses 24:481–495.Google Scholar
  11. Vogt, R. G., Rogers, M. E., Franco, M. D., and Sun, M. 2002. A comparative study of odorant binding protein genes: differential expression of the PBPl-GOBP2 gene cluster in Manduca Sexta (Lepidoptera) and the organization of OBP genes in Drosophila melanogaster (Diptera). J. Exp. Biol. 205:719–744.Google Scholar

Copyright information

© Plenum Publishing Corporation 2002

Authors and Affiliations

  • Richard G. Vogt
    • 1
  1. 1.Department of Biological SciencesUniversity of South CarolinaColumbia

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