Photosynthesis Research

, Volume 74, Issue 2, pp 135–141 | Cite as

Structural factors which control the position of the Qy absorption band of bacteriochlorophyll a in purple bacterial antenna complexes

  • R.J. Cogdell
  • T.D. Howard
  • N.W. Isaacs
  • K. McLuskey
  • A.T. Gardiner


This paper presents a concise review of the structural factors which control the energy of the Qy absorption band of bacteriochlorophyll a in purple bacterial antenna complexes. The energy of these Qy absorption bands is important for excitation energy transfer within the bacterial photosynthetic unit.

bacteriochlorophyll light-harvesting complexes purple photosynthetic bacteria 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aargaard J and Sistrom W (1972) Control of synthesis of reaction centre bacteriochlorophyll in photosynthetic bacteria. Photochem Photobiol 15: 200–225Google Scholar
  2. Alden R, Johnson E, Nagarajan V, Parson W, Law C and Cogdell R (1997) Calculations of spectroscopic properties of the LH2 bacteriochlorophyll-protein antenna complex from Rps. acidophila. J Phys Chem B 101: 4667–4680CrossRefGoogle Scholar
  3. Bissig I, Wagner-Huber R, Brunisholz R and Zuber H (1990) Multiple antenna complexes in various purple photosynthetic bacteria. In: Drews G and Dawes E (eds) Molecular Biology of Membrane-Bound Complexes in Phototrophic Bacteria, pp 199–210. Plenum Press, New YorkGoogle Scholar
  4. Cogdell RJ and Lindsay JG (1998) Can photosynthesis provide a 'biological blueprint' for the design of novel solar cells? Trends Biotechnol 16: 521–527CrossRefGoogle Scholar
  5. Cogdell RJ, Durant I, Valentine J, Lindsay JG and Schmidt K (1983) The isolation and partial characterization of the light-harvesting pigment-protein complement of Rhodopseudomonas acidophila. Biochim Biophys Acta 722: 427–435CrossRefGoogle Scholar
  6. Cogdell RJ, Isaacs NW, Howard TD, McLuskey K, Fraser N and Prince SM (1999) How photosynthetic bacteria harvest solar energy. J Bacteriol 181: 3869–3879PubMedGoogle Scholar
  7. Drews G (1985) Structure and functional-organization of lightharvesting complexes and photochemical-reaction centers in membranes of phototrophic bacteria. Microbiol Rev 49: 59–70PubMedGoogle Scholar
  8. Eccles J and Honig B (1983) Charged amino-acids as spectroscopic determinants for chlorophyll in vivo. Proc Natl Acad Sci USA 80: 4959–4962PubMedCrossRefGoogle Scholar
  9. Fiedor L, Scheer H, Hunter CN, Tschirschwitz F, Voigt B, Ehlert J, Nibbering E, Leupold D and Elsaesser T (2000) Introduction of a 60 fs deactivation channel in the photosynthetic antenna LH1 by Ni-bacteriopheophytin a. Chem Phys Lett 319: 145–152CrossRefGoogle Scholar
  10. Fowler G, Visschers R, Grief G, van Grondelle R and Hunter C (1992) Genetically modified photosynthetic antenna complexes with blueshifted absorbance bands. Nature 355: 848–850PubMedCrossRefGoogle Scholar
  11. Fowler G, Sockalingum G, Robert B and Hunter C (1994) Blue shifts in bacteriochlorophyll absorbance correlate with changed hydrogen bonding patterns in light-harvesting 2 mutant of Rhodobacter sphaeroides with alterations at ?-Tyr-44 and ?-Tyr. Biochem J 299: 695–700PubMedGoogle Scholar
  12. Gentemann S, Nelson NY, Jaquinod L, Nurco DJ, Leung SH, Medforth CJ, Smith KM, Fajer J and Holten D (1997) Variations and temperature dependence of the excited state properties of conformationally and electronically perturbed zinc and free base porphyrins. J Phys Chem B 101: 1247–1254CrossRefGoogle Scholar
  13. Gudowska-Nowak E, Newton MD and Fajer F (1990) Conformational and environmental effects on bacteriochlorophyll optical spectra: correlations of calculated spectra with structural results. J Phys Chem 94: 5795–5801CrossRefGoogle Scholar
  14. Herek JL, Fraser NJ, Pullerits T, Martinsson P, Polivka T, Scheer H, Cogdell RJ and Sundstrom V (2000) B800?B850 energy transfer mechanism in bacterial LH2 complexes investigated by B800 pigment exchange. Biophys J 78: 2590–2596PubMedGoogle Scholar
  15. Hiraishi A and Shimada K (2001) Aerobic anoxygenic photosynthetic bacteria with zinc-bacteriochlorophyll. J Gen Appl Microbiol 47: 161–180PubMedCrossRefGoogle Scholar
  16. Hu X and Schulten K (1998) Model for the light-harvesting complex I (B875) of Rhodobacter sphaeroides. Biophys J 75: 683–694PubMedCrossRefGoogle Scholar
  17. Hunter C (1995) Genetic manipulation of the antenna complexes of purple bacteria. In: Blankenship R, Madigan M and Bauer C (eds) Anoxygenic Photosynthetic Bacteria, pp 473–501. Kluwer Academic Publishers, Dordrecht, The NetherlandsGoogle Scholar
  18. Karrasch S, Bullough P and Ghosh R (1995) The 8.5 Å projection map of the light-harvesting complex I from Rhodospirillium rubrum. EMBO J 14: 631–638PubMedGoogle Scholar
  19. Koepke J, Hu X, Muenke C, Schulten K and Michel H (1996) The crystal structure of the light-harvesting complex ll (B800-850) from Rhodospirillium molischianum. Structure 4: 581–597PubMedCrossRefGoogle Scholar
  20. Koolhaas MHC, van der Zwan G, Frese RN and van Grondelle R (1997) Red shift of the zero crossing in the CD spectra of the LH2 antenna complex of Rhodopseudomonas acidophila: a structurebased study. J Phys Chem B 101: 7262–7270CrossRefGoogle Scholar
  21. Kraulis PJ (1991) Molscript-a program to produce both detailed and schematic plots of protein structures. J Appl Cryst 24: 946–950CrossRefGoogle Scholar
  22. Lapouge K, Naveke A, Robert B, Scheer H and Sturgis JN (2000) Exchanging cofactors in the core antennae from purple bacteria: structure and properties of Zn-bacteriopheophytin-containing LH1. Biochemistry 39: 1091–1099PubMedCrossRefGoogle Scholar
  23. McDermott G (1997) Structural studies on an integral membrane light-harvesting complex. PhD Thesis, University of Glasgow, UKGoogle Scholar
  24. McDermott G, Prince S, Freer A, Hawthornwaite-Lawless A, Papiz M, Cogdell R and Isaacs N (1995) Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria. Nature 374: 517–521CrossRefGoogle Scholar
  25. McLuskey K (1999) Crystallographic studies on integral membrane light-harvesting complexes from photosynthetic bacteria. PhD Thesis, University of Glasgow, UKGoogle Scholar
  26. McLuskey K, Prince SM, Cogdell RJ and Isaacs NW (2001) The crystallographic structure of the B800-820 LH3 light-harvesting complex from the purple bacteria Rhodopseudomonas acidophila strain 7050. Biochemistry 40: 8783–8789PubMedCrossRefGoogle Scholar
  27. Papiz M, Prince S, Hawthornwaite-Lawless A, McDermott G, Freer A, Isaacs N and Cogdell R (1996) How photosynthesis works in purple bacteria: a circular argument. TIPS 1: 198–206Google Scholar
  28. Prince S, Papiz M, Freer A, McDermott G, Hawthornwaite-Lawless A, Cogdell R and Isaacs N (1997) Apoprotein structure in LH2 complex from Rhodopseudomonas acidophila strain 10050: modular assembly and protein pigment interactions. J Mol Biol 268: 1–12CrossRefGoogle Scholar
  29. Sauer K, Cogdell R, Prince S, Freer A, Isaacs N and Scheer H (1996) Structure-based calculations of the optical spectra of the LH2 bacteriochlorophyll-protein complex from Rhodopseudomonas acidophila. Photochem Photobiol 64: 564–576Google Scholar
  30. Scheer H and Struck A (1993) Bacterial reaction centres with modified tetrapyrrole chromophores. In: Deisenhofer J and Norris J (eds) The Photosynthetic Reaction Centre, pp 157–192. Academic Press, LondonGoogle Scholar
  31. Scholes G, Gould I, Cogdell R and Fleming G (1999) Ab initio Molecular orbital calculations of electronic couplings in the LH2 bacterial light-harvesting complex of Rps. acidophila. J Phys Chem B 103: 2543–2553CrossRefGoogle Scholar
  32. Sistrom W (1978) Control of antenna pigment components. In: Clayton R and Sistrom W (eds) The Photosynthetic Bacteria, pp 841–848. Plenum Press, New YorkGoogle Scholar
  33. Sturgis J, Jirsakova V, Reiss-Husson F, Cogdell R and Robert B (1995) Structure and properties of bacteriochlorophyll binding site in peripheral light-harvesting complexes of purple bacteria. Biochemistry 34: 517–523PubMedCrossRefGoogle Scholar
  34. Swallen S, Kopelman R and IMoore J (1998) Excited state dynamics in organic dendrimer supermolecules. Exciton processes in condensed matter EXCON '98 85-92Google Scholar
  35. Tronrud DE, Schmid MF and Matthews BW (1986) Structure and X-ray amino-acid-sequence of a bacteriochlorophyll-a protein from Prosthecochloris-aestuarii refined at 1.9 Å resolution. J Mol Biol 188: 443–454PubMedCrossRefGoogle Scholar
  36. van Grondelle R, Dekker J, Gillbro T and Sundstrom V (1994) Energy transfer and trapping in photosynthesis. Biochim Biophys Acta 1187: 1–65CrossRefGoogle Scholar
  37. Zuber H and Brunisholz R (1991) Structure and function of antenna polypeptides and chlorophyll-protein complexes: principles and variability. In: Scheer H (eds) Chlorophylls, pp 627–704. CRC Press, Boca Raton, FloridaGoogle Scholar
  38. Zuber H and Cogdell R (1995) Structure and organisation of purple bacterial antenna complexes. In: Blankenship R, Madigan M and Bauer C (eds) Anoxygenic Photosynthetic Bacteria, pp 315–348. Kluwer Academic Publishers, Dordrecht, The NetherlandsGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • R.J. Cogdell
    • 1
  • T.D. Howard
    • 1
  • N.W. Isaacs
    • 2
  • K. McLuskey
    • 2
  • A.T. Gardiner
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, IBLSUniversity of GlasgowGlasgowUK
  2. 2.Department of ChemistryUniversity of GlasgowGlasgowUK

Personalised recommendations