Plant Growth Regulation

, Volume 38, Issue 1, pp 15–20 | Cite as

The biosynthetic pathway for indole-3-acetic acid changes during tomato fruit development

  • Ephraim Epstein
  • Jerry D. Cohen
  • Janet P. Slovin


Phytohormone metabolism during fruit ripening is critical to the controlof this developmental process, yet we know little about pathways for theproduction of many of these signaling compounds. Using stable isotope labelingin both an in vitro aseptic tomato fruit culture systemanddetached greenhouse-grown tomato fruit, we have shown by mass spectral analysisthat tomato uses the tryptophan-independent pathway to produce IAA fromanthranilate or indole. We also show that there is a developmental switch fromtryptophan utilization to tryptophan-independent production that occurs betweenmature green and red-ripe stages of fruit development. Moreover, this pathwayswitch does not appear to be associated with ripening per se in that fruit fromneverripe tomato plants also utilize the tryptophanindependent pathway.

Auxin Developmental regulation Fruit ripening Lycopersicon esculentum L. Neverripe Phytohormones Stable isotopes 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Ephraim Epstein
    • 1
  • Jerry D. Cohen
    • 2
  • Janet P. Slovin
    • 3
  1. 1.Department of Plant ScienceCook College, Rutgers-The State University of New JerseyNew BrunswickUSA
  2. 2.Department of Horticultural ScienceUniversity of MinnesotaSaint PaulUSA
  3. 3.Fruit LaboratoryAgricultural Research Service, United States Department of AgricultureBeltsvilleUSA

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