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Chromosome Research

, Volume 10, Issue 7, pp 607–613 | Cite as

Genetic load caused by variation in the amount of rDNA in a wasp

  • S. M. S. R. Araújo
  • C. C. Silva
  • S. G. Pompolo
  • F. Perfectti
  • J. P. M. Camacho
Article

Abstract

Extensive variation in the size of the short (heterochromatic) arm of chromosome 14 was found in the wasp Trypoxylon (Trypargilum) albitarse. Ten different variants were differentiated by size and C-banding pattern. Fluorescent in-situ hybridization (FISH) revealed that ribosomal DNA in this species is clustered in the darkly C-banded parts of the heterochromatic short arm of chromosome 14. On this basis, we got an indirect estimate of the amount of rDNA from the area of these dark C-bands. The significant absence in males of the three chromosome variants with lower amounts of rDNA indicates that these three variants are lethal in this sex, and suggests the existence of a threshold marking the minimum amount of rDNA which is tolerable in haploidy. This implies about 4% genetic load in the population caused by variation in rDNA amount.

genetic load Hymenoptera nucleolus organiser rDNA Trypoxylon albitarse 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • S. M. S. R. Araújo
    • 1
    • 2
  • C. C. Silva
    • 1
  • S. G. Pompolo
    • 1
  • F. Perfectti
    • 2
  • J. P. M. Camacho
    • 2
  1. 1.Departamento de Biologia CelularUniversidade Estadual de Campinas (UNICAMP)Campinas, SPBrazil
  2. 2.Departamento de Genética, Facultad de CienciasUniversidad de GranadaGranadaSpain

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