Biotechnology Letters

, Volume 24, Issue 22, pp 1913–1917

Fast and efficient adsorption/desorption of protein by a novel magnetic nano-adsorbent

  • Min-Hung Liao
  • Dong-Hwang Chen


A novel magnetic nano-adsorbent was prepared by covalently binding polyacrylic acid (PAA) on Fe3O4 superparamagnetic nanoparticles (13.2 nm) via carbodiimide activation. The maximum weight ratio of PAA to Fe3O4 was 0.12 (i.e., average of two PAA molecules on a magnetic nanoparticle). The magnetic nano-adsorbent possessed a high ionic exchange capacity of 1.64 meq g−1 and was efficient for the recovery of lysozyme. The lysozyme could be completely adsorbed in 0.1 M phosphate buffer at pH 3–5 and completely desorbed in NaSCN solution (>1 M) within 1 min, and retained 95% activity after adsorption/desorption. In addition, the adsorption behavior followed the Langmuir adsorption isotherm with a maximum adsorption amount of 0.224 mg mg−1 and a Langmuir adsorption equilibrium constant of 10 ml mg−1 at 25 °C. The change of enthalpy at 15–35 °C was −4.2 kJ ml mol−1 mg−1.

adsorption magnetic nanoparticles nano-adsorbent PAA 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bajpai AK (2000) Adsorption of bovine serum albumin onto glass powder surfaces coated with polyvinyl alcohol. J. Appl. Polym. Sci. 78: 933-940.Google Scholar
  2. Chen DH, Wu SH (2000) Synthesis of nickel nanoparticles in waterin-oil microemulsions. Chem. Mater. 12: 1354-1360.Google Scholar
  3. Edelstein AS, Cammarata RC (1996) Nanomaterials: Synthesis, Properties and Applications. Bristol, UK: Institute of Physics Publishing.Google Scholar
  4. Harland CE (1994) Ion Exchange: Theory and Practice. Cambridge, UK: The Royal Society of Chemistry.Google Scholar
  5. Khng HP, Cunliffe D, Davies S, Turner NA, Vulfson EN (1998) The synthesis of sub-micron magnetic particles and their use for preparative purification of protein. Biotechnol. Bioeng. 60: 419-424.Google Scholar
  6. Liao MH, Chen DH (2001) Immobilization of yeast alcohol dehydrogenase on magnetic nanoparticles for improving its stability. Biotechnol. Lett. 23: 1723-1727.Google Scholar
  7. Lu Y, Yin Y, Mayers BT, Xia Y (2002) Modifiyng the surface properties of superparamagnetic iron oxide nanoparticles through a sol-gel approach. Nano Lett. 2: 183-186.Google Scholar
  8. Ngah WSW, Endud CS, Mayanar R (2002) Removal of copper( II) ions from aqueous solution onto chitosan and cross-linked chitosan beads. React. Funct. Polym. 50: 181-190.Google Scholar
  9. Pieters BR, Williams RA, Webb C (1992) Magnetic carrier technology. In: Williams RA, ed. Colloid and Surface Engineering: Applications in the Process Industries. Oxford: Butterworth-Heinemann, pp. 248-286.Google Scholar
  10. Ruckenstein E, Zeng X (1997) Macroporous chitin affinity membranes for lysozyme separation. Biotechnol. Bioeng. 56: 610-617.Google Scholar
  11. Šafařrík I, Šafařríková M (1999) Use of magnetic techniques for the isolation of cells. J. Chromatogr. B722: 33-53.Google Scholar
  12. Suzawa T, Shirahama H (1991) Adsorption of plasma proteins onto polymer lattices. Adv. Colloid Interface Sci. 35: 139-172.Google Scholar
  13. Wahlgren MC, Arnebrant T, Paulsson MA (1993) The adsorption from solutions of β-lactoglobulin mixed with lactoferrin or lysozyme onto silica and methylated silica surfaces. J. Colloid Interface Sci. 153: 46-53.Google Scholar
  14. Xue B, Sun Y (2002) Fabrication and characterization of a rigid magnetic matrix for protein adsorption. J. Chromatogr. A947: 185-193.Google Scholar
  15. Zadow JG, Hill RD (1975) The precipitation of proteins by carboxymethyl cellulose. J. Dairy Res. 42: 267-275.Google Scholar
  16. Zhang Y, Kohler N, Zhang M (2002) Surface modification of superparamagnetic magnetic nanoparticles and their intracellular uptake. Biomaterials 23: 1553-1561.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Min-Hung Liao
    • 1
  • Dong-Hwang Chen
    • 1
  1. 1.Department of Chemical EngineeringNational Cheng Kung UniversityTainanTaiwan R.O.C

Personalised recommendations