Breast Cancer Research and Treatment

, Volume 76, Issue 3, pp 195–201

(−)-Epigallocatechin (EGC) of Green Tea Induces Apoptosis of Human Breast Cancer Cells But Not of their Normal Counterparts

  • David Vergote
  • Cécile Cren-Olivé
  • Valérie Chopin
  • Robert-Alain Toillon
  • Christian Rolando
  • Hubert Hondermarck
  • Xuefen Le Bourhis
Article

DOI: 10.1023/A:1020833410523

Cite this article as:
Vergote, D., Cren-Olivé, C., Chopin, V. et al. Breast Cancer Res Treat (2002) 76: 195. doi:10.1023/A:1020833410523

Abstract

(−)-Epigallocatechin (EGC), one of green tea polyphenols, has been shown to inhibit growth of cancer cells. However its mechanism of action is poorly known. We show here that EGC strongly inhibited the growth of breast cancer cell lines (MCF-7 and MDA-MB-231) but not that of normal breast epithelial cells. The inhibition of breast cancer cell growth was due to an induction of apoptosis, without any change in cell cycle progression. MCF-7 cells are known to express a wild-type p53 whereas MDA-MB-231 cells express a mutated p53. The fact that EGC induced apoptosis in both these cell lines suggests that the EGC-triggered apoptosis is independent of p53 status. Moreover, neutralizing antibodies against the death receptor Fas and inhibitors of caspases, such as caspase-8 and -10, efficiently inhibited the EGC-triggered apoptosis. In addition, immunoblotting revealed that EGC treatment was correlated with a decrease in Bcl-2 and an increase in Bax level. These results suggest that EGC-triggered apoptosis in breast cancer cells requires Fas signaling.

apoptosis Bcl-2 breast cancer Fas signaling flavan-3-ols tea polyphenols 

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • David Vergote
    • 1
  • Cécile Cren-Olivé
    • 2
  • Valérie Chopin
    • 1
  • Robert-Alain Toillon
    • 1
  • Christian Rolando
    • 2
  • Hubert Hondermarck
    • 1
  • Xuefen Le Bourhis
    • 1
  1. 1.Laboratoire de Biologie du Développement (UPRES-EA 1033), Equipe PolyphénolsUniversité des Sciences et Technologies de LilleVilleneuve d'AscqFrance
  2. 2.Laboratoire de Chimie Organique et Macromoléculaire (UPRESA-8009 CNRS), Equipe PolyphénolsUniversité des Sciences et Technologies de LilleVilleneuve d'AscqFrance

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