Metabolic Brain Disease

, Volume 14, Issue 2, pp 117–124

Targeted Disruption of the bcl-2 Gene in Mice Exacerbates Focal Ischemic Brain Injury

  • Ryuji Hata
  • Frank Gillardon
  • Theologos M. Michaelidis
  • Konstantin-Alexander Hossmann


Neuronal death after brain ischemia is mainly due to necrosis but there is also evidence for involvement of apoptosis. To test the importance of apoptosis, we investigated the effect of targeted disruption of the apoptosis-suppressive gene bcl-2 on the severity of ischemic brain injury. Transient focal ischemia for 1 hour was induced by occlusion of the middle cerebral artery in homozygous (n=7) and heterozygous (n = 6) bcl-2 knockout mice as well as in their wildtype littermates (n=5). Bcl-2 ablation did not influence cerebral blood flow but it significantly increased infarct size and neurological deficit score at 1 day after reperfusion in a gene-dose dependent manner. The exacerbation of tissue damage in the absence of Bcl-2 underscores the importance of apoptotic pathways for the manifestation of ischemic injury after transient vascular occlusion.

Cerebral ischemia focal ischemia mutant mice bcl-2 apoptosis 


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Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • Ryuji Hata
    • 1
    • 2
  • Frank Gillardon
    • 1
  • Theologos M. Michaelidis
    • 3
  • Konstantin-Alexander Hossmann
    • 1
  1. 1.Department of Experimental NeurologyMax-Planck-Institute for Neurological ResearchCologneGermany
  2. 2.BF Research InstituteOsakaJapan
  3. 3.Division of Molecular Biology of the Cell IIGerman Cancer Research CenterHeidelbergGermany
  4. 4.Max-Planck-Institut für neurologische Forschung, Abteilung für experimentelle NeurologieCologneGermany

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