, Volume 128, Issue 1, pp 9–17 | Cite as

Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding

  • M. Pradeep Reddy
  • N. Sarla
  • E.A. Siddiq


Inter simple sequence repeat (ISSR)-PCR is a technique, which involvesthe use of microsatellite sequences as primers in a polymerase chainreaction to generate multilocus markers. It is a simple and quick methodthat combines most of the advantages of microsatellites (SSRs) andamplified fragment length polymorphism (AFLP) to the universality ofrandom amplified polymorphic DNA (RAPD). ISSR markers are highlypolymorphic and are useful in studies on genetic diversity, phylogeny, genetagging, genome mapping and evolutionary biology. This review providesan overview of the details of the technique and its application in geneticsand plant breeding in a wide range of crop plants.

anchored primer DNA marker genome mapping gene tagging genetic diversity ISSR-PCR 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • M. Pradeep Reddy
    • 1
  • N. Sarla
    • 1
  • E.A. Siddiq
    • 1
  1. 1.Directorate of Rice ResearchRajendranagar, HyderabadIndia

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