Genetic Resources and Crop Evolution

, Volume 49, Issue 4, pp 385–397 | Cite as

Variation in chromosome numbers and nuclear DNA contents in genetic resources of Lactuca L. species (Asteraceae)

  • Ivana Doležalová
  • Aleš Lebeda
  • Josef Janeček
  • Jarmila Číhalíková
  • Eva Křístková
  • Olga Vránová
Article

Abstract

Fifty accessions of 25 Lactuca species,L. serriola ×L. sativa and Mycelismuralis were analyzed for chromosome number and relative DNA amountvariation. In the majority of Lactuca species studiedchromosome counts, as earlier reported (n = 8, 9, 17), were verified; however,for L. dregeana andL. homblei (probablyL. schweinfurthii orL. longespicata) the chromosome number(n = 9) was determined for the first time. Relative nuclear DNA content,estimated by using flow cytometry (DAPI staining), showed that 2C DNA contentranged from 2.02 pg in L.capensis to 17.96 pg inL. canadensis. Statistical and clusteranalysis of data based on relative nuclear DNA contents correspond fairly wellwith recently accepted taxonomic classification of the genusLactuca. However, the position of certain species as wellas clarification of taxonomic determinations of someLactuca accessions needs further examination.

Asteraceae Chromosome number Flow cytometry Gene pools Relative nuclear DNA content Taxonomy 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Ivana Doležalová
    • 1
  • Aleš Lebeda
    • 1
  • Josef Janeček
    • 2
  • Jarmila Číhalíková
    • 3
  • Eva Křístková
    • 4
  • Olga Vránová
    • 5
  1. 1.Faculty of Science, Department of BotanyPalacký UniversityOlomouc-HoliceCzech Republic
  2. 2.Plant Breeding StationHladké ŽivoticeCzech Republic
  3. 3.Institute of Experimental BotanyCzech Academy of ScienceOlomoucCzech Republic
  4. 4.Department of GenebankResearch Institute of Crop Production PrahaOlomouc-HoliceCzech Republic
  5. 5.Pedagogical Faculty, Department of Biology and CultivationPalacký UniversityOlomoucCzech Republic

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