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Microbiology

, Volume 71, Issue 5, pp 525–531 | Cite as

Involvement of Microbial Alkyl Hydroxybenzenes in the Regulation of Autolytic Degradation of Yeast Cells

  • T. A. Karpekina
  • I. Yu. Stepanenko
  • E. I. Krylova
  • A. N. Kozlova
  • I. M. Gracheva
  • G. I. El'-Registan
Article

Abstract

A comparative study was performed of the processes of autolytic degradation of the cells of Saccharomyces cerevisiae and Schizosaccharomyces pombe under conditions simulating the phase of cell death in microbial cultures: (1) during autolysis induced by oleic acid, which is the chemical analogue of factors d2 (autolysis autoinducer), (2) under the effect of extracellular microbial proteinases (enzymatic lysis), and (3) under the concomitant effect of the enzymes of the endogenous autolytic complex and exogenous proteinases (heterolysis). Regulatory mechanisms controlling the rate and profundity of autolysis were elucidated, relying on the stabilization of hydrolytic enzymes and enhancement of their activity in their complexes with a chemical analogue of microbial autoregulatory factors d1, which belong to alkylhydroxybenzenes and fulfill functions of chemical chaperones. The changes in the activity of proteinases and enzymes of the autolytic complex were shown to be dependent on the concentration of the analogue at the moment of complex formation.

autolysis chemical chaperons enzyme stabilization stimulation of catalytic activity 

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Copyright information

© MAIK “Nauka/Interperiodica” 2002

Authors and Affiliations

  • T. A. Karpekina
    • 1
  • I. Yu. Stepanenko
    • 2
  • E. I. Krylova
    • 2
  • A. N. Kozlova
    • 2
  • I. M. Gracheva
    • 1
  • G. I. El'-Registan
    • 1
  1. 1.Moscow State University of the Food IndustryMoscowRussia
  2. 2.Institute of MicrobiologyRussian Academy of SciencesMoscowRussia

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