Advertisement

Antonie van Leeuwenhoek

, Volume 81, Issue 1–4, pp 263–270 | Cite as

Methanogenium marinum sp. nov., a H2-using methanogen from Skan Bay, Alaska, and kinetics of H2 utilization

  • Song C. Chong
  • Yitai Liu
  • Michael Cummins
  • David L. Valentine
  • David R. Boone
Article

Abstract

A methanogen, strain AK-1, was isolated from permanently cold marine sediments, 38- to 45-cm below the sediment surface at Skan Bay, Alaska. The cells were highly irregular, nonmotile coccoids (diameter, 1 to 1.2 μm), occurring singly. Cells grew by reducing CO2 with H2 or formate as electron donor. Growth on formate was much slower than that on H2. Acetate, methanol, ethanol, 1- or 2-propanol, 1- or 2-butanol and trimethylamine were not catabolized. The cells required acetate, thiamine, riboflavin, a high concentration of vitamin B12, and peptones for growth; yeast extract stimulated growth but was not required. The cells grew fastest at 25 °C (range 5 °C to 25 °C), at a pH of 6.0 – 6.6 (growth range, pH 5.5 – 7.5), and at a salinity of 0.25 – 1.25 M Na+. Cells of this and other H2-using methanogens from saline environments metabolized H2 to a very low threshold pressure (less than 1 Pa) that was dependent on the methane partial pressure. We propose that the threshold pressure may be limited by the energetics of catabolism. The sequence of the 16S rDNA gene of strain AK-1 was most similar (98%) to the sequences of Methanogenium cariaci JR-1 and Methanogenium frigidum Ace-2. DNA–DNA hybridization between strain AK-1 and these two strains showed only 34.9% similarity to strain JR-1 and 56.5% similarity to strain Ace-2. These analyses indicated strain AK-1 should be classified as a new species within the genus Methanogenium. Phenotypic differences between strain AK-1 and these strains (including growth temperature, salinity range, pH range, and nutrient requirements) support this. Therefore, a new species, Methanogenium marinum, is proposed with strain AK-1 as type strain.

marine sediments methanogen Methanogenium Methanogenium marinum psychrophile 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alperin MJ & Reeburgh WS (1992) Organic carbon remineralization and preservation in sediments of Skan Bay, Alaska. In: Whelan JK & Farrington JW (Eds) Ogranic Matter Productivity, Accumulation, and Preservation in Recent and Ancient Sediments. Vol. XII, p. 533. Columbia University Press, New York.Google Scholar
  2. Balch WE, Fox GE (1979) Methanogens: reevaluation of a unique biological group. Microbiol. Rev. 43: 260–296.PubMedGoogle Scholar
  3. Balch WE & Wolfe RS (1976) New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HSCoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl. Environ. Microbiol. 32: 781–791.PubMedGoogle Scholar
  4. Boone DR, & Castenholz R (Eds) (1999) Bergey's Manual of Systematic Bacteriology. Springer-Verlag, Heidelberg, Germany.Google Scholar
  5. Boone DR & Johnson RL (1989) Diffusion of hydrogen to methanogens and effects on measurements of enzyme kinetics. Workshop on Microbiology of Landfills, Beaverton, Oregon.Google Scholar
  6. Boone DR & Whitman WB (1988) Proposal of minimal standards for describing new taxa of methanogenic bacteria. Int. J. Syst. Bacteriol. 38: 212–219.Google Scholar
  7. Cashion P & Holder-Franklin MA (1977) A rapid method for base ratio determination of bacterial DNA. Anal. Biochem. 81: 461–466.PubMedCrossRefGoogle Scholar
  8. Conrad R (1999) Contribution of hydrogen to methane production and control of hydrogen concentrations in methanogenic soils and sediments. FEMS Microbiol. Ecol. 28: 193–202.CrossRefGoogle Scholar
  9. De Ley J & Cattoir H (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12: 133–142.PubMedCrossRefGoogle Scholar
  10. Escara JF & Hutton JR (1980) Thermal stability and renaturation of DNA in dimethylsulphoxide solutions: acceleration of renaturation rate. Biopolymers 19: 1315–1327.PubMedCrossRefGoogle Scholar
  11. Franzmann PD & Liu Y (1997) Methanogenium frigidum sp. nov., a psychrophilic, H2-using methanogen from Ace Lake, Antarctica. Int. J. Syst. Bacteriol. 47: 1068–1072.PubMedGoogle Scholar
  12. Gompertz B (1825) On the nature of the function expressive of the law of human mortality and on a new mode of determining the value of life contingencies. Philos. Trans. R. Soc. London 115: 513–585.Google Scholar
  13. Hungate RE (1969) A roll tube method for cultivation of strict anaerobes. In: Norris JR & Ribbons DW (Eds) Methods in Microbiology Vol 3B (pp 117–132). Academic Press, New York.Google Scholar
  14. Huss V A R & Festl H (1983) Studies on the spectrometric determination of DNA hybridization from renaturation rates. J Syst. Appl. Microbiol. 4: 184–192.Google Scholar
  15. Jahnke K-D (1992) Basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrometer on a PC/XT/AT type personal computer. J. Microbiol. Meth. 15: 61–73.CrossRefGoogle Scholar
  16. Jones WJM & Paynter JB (1983) Characterization of Methanococcus maripaludis sp. nov., a new methanogen isolated from salt marsh sediment. Arch. Microbiol. 135: 91–97.CrossRefGoogle Scholar
  17. Lovley DR & Goodwin S (1988) Hydrogen concentrations as an indicator of the predominant terminal electron-accepting reactions in aquatic sediments. Geochim. Cosmochim. Acta 52: 2993–3003.CrossRefGoogle Scholar
  18. Maestrojuán GM & Boone DR (1991) Characterization of Methanosarcina barkeri MST and 227, Methanosarcina mazei S-6T, and Methanosarcina vacuolata Z-761T. Int. J. Syst. Bacteriol. 41: 267–274.Google Scholar
  19. Maestrojuán GM, & Boone DR (1990) 'Transfer of Methanogenium bourgense, Methanogenium marisnigri, Methanogenium olentangyi, and Methanogenium thermophilicum to the genus Methanoculleus gen. nov., emendation of Methanoculleus marisnigri, and description of new strains of Methanoculleus bourgense and Methanoculleus marisnigri. Int. J. Syst. Bacteriol. 40: 117–122.Google Scholar
  20. Munson MA & Nedwell DB (1997) Phylogenetic diversity of Archaea in sediment samples from a coastal salt marsh. Appl. Environ. Microbiol. 63(12): 4729–4733.PubMedGoogle Scholar
  21. Powell GE (1983) Interpreting gas kinetics of batch culture. Biotechnol. Lett. 5: 437–440.CrossRefGoogle Scholar
  22. Romesser JA & Wolfe RS (1979) Methanogenium, a new genus of marine methanogenic bacteria, and characterization of Methanogenium cariaci sp. nov. and Methanogenium marisnigri sp. nov. Arch. Microbiol. 121: 147–153.CrossRefGoogle Scholar
  23. Stadtman TC & Barker HA (1951) Studies on the methane fermentation: X. A new formate-decomposing bacterium, Methanococcus vannielii. J. Bacteriol. 62: 269–280.PubMedGoogle Scholar
  24. Valentine DL & Reeburgh WS (2000) New perspectives on anaerobic methane oxidation. Environ. Microbiol. 2: 477–484.PubMedCrossRefGoogle Scholar
  25. Vetriani C, & Jannasch HW (1999) Population structure and phylogenetic characterization of marine benthic Archaea in deep-dea sediments. Appl. Environ. Microbiol. 65(10): 4375–4384.PubMedGoogle Scholar
  26. Wayne LG & Brenner DJ (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37: 463–464.CrossRefGoogle Scholar
  27. Zhang J-Z & Millero FJ (1993) The chemistry of anoxic waters in the Cariaco Trench. Deep-Sea Res. 39: 1023–1041.Google Scholar
  28. Zwietering MH, & Jongenburger I (1990) Modeling of the bacterial growth curve. Appl. Environ. Microbiol. 56: 1875–1881.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Song C. Chong
    • 1
  • Yitai Liu
    • 1
  • Michael Cummins
    • 1
  • David L. Valentine
    • 2
  • David R. Boone
    • 1
  1. 1.Department of BiologyPortland State UniversityPortlandUSA, and
  2. 2.Department of Earth System ScienceUniversity of CaliforniaIrvineUSA

Personalised recommendations