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Biodegradation

, Volume 13, Issue 2, pp 131–140 | Cite as

Priming effect as determined by adding 14C-glucose to modified controlled composting test

  • Marja Tuomela
  • Annele Hatakka
  • Sari Karjomaa
  • Merja Itävaara
Article

Abstract

The development of new biodegradable packaging materials, especially biodegradable plastics, has created a need for biodegradability testing. The European standard for controlled composting test was used in this study for assessing if the addition of a test material results in excess CO2 production in compost. This effect, designated as the priming effect, would give an erroneous result for biodegradation, which is based on CO2 formation from the test material. Glucose was selected as a test substrate because it is the degradation product of starch and cellulose, which are major compounds of many packaging materials. Both 14C-glucose and non-labelled glucose was applied to nine compost samples of variable stability and agefrom two weeks to 1.5 years. CO2 and 14CO2 evolution were measured during the incubation. Biodegradation of glucose in unstable composts (age leq6 months) was negative and 14CO2 evolution was poor, although the respective composts without glucose produced relatively high amounts of CO2. It was concluded that a negative priming effect was observed in unstable composts, in which glucose remained mostly non-degraded and apparently inhibited the mineralization of native organic matter in the compost. In stable composts (age ≥6 months), biodegradation of glucose was high and approximately equal to 14C-glucose mineralization, i.e., the composts showed no priming effect. Young composts were unsuitable for controlled composting test due to lack of stability. It is important to ensure that the compost inoculum used for the test is sufficiently stable.

biodegradation compost compost maturity glucose priming effect 

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References

  1. Bingeman CW, Varner JE& Martin WP (1953) The effect of the addition of organic materials on the decomposition of an organic soil. Soil Sci. Soc. Am. Proc. 17: 34-38Google Scholar
  2. Carpenter-Boggs L, Kennedy AC& Reganold JP (1998) Use of phospholipid fatty acids and carbon source utilization patterns to track microbial community succession in developing compost. Appl. Environ. Microbiol. 64: 4062-4064Google Scholar
  3. Chantigny MH, Angers DA, Prévost D, Simard RR& Chalifour F-P (1999) Dynamics of soluble organic C and C mineralization in cultivated soils with varying N fertilization. Soil Biol. Biochem. 31: 543-550Google Scholar
  4. Dalenberg JW& Jager G (1981) Priming effect of small glucose additions to 14C-labelled soil. Soil Biol. Biochem. 13: 219-223Google Scholar
  5. Degli-Innocenti F, Tosin M& Bastioli C (1998) Evaluation of the biodegradation of starch and cellulose under controlled composting conditions. J. Environ. Polymer Degrad. 6: 197-202Google Scholar
  6. European Standard prEN 14046 (2000) Packaging-evaluation of the ultimate aerobic biodegradability and disintegradation of packaging material under controlled composting conditions-method by analysis of released carbon dioxide.Google Scholar
  7. Ezelin K, Brun G, Kaemmerer M& Revel JC (1996) Glucose influence on the asymbiotic nitrogen fixation during lignocellulosic waste composting. In: Bertoldi M, Sequi P, Lemmes B& Papi T (Eds) European Commission International Symposium. The Science of Composting (pp 137-148). Blackie Academic&Professional, LondonGoogle Scholar
  8. Forster JC, Zech W& Würdinger E (1993) Comparison of chemical and microbiological methods for the characterization of the maturity of composts from contrasting sources. Biol. Fertil. Soils 16: 93-99Google Scholar
  9. Gonzáles-Vila FJ, Almendros G& Madrid F (1999) Molecular alterations of organic fractions from urban waste in the course of composting and their further transformation in amended soil. Sci. Total Environ. 236: 215-229Google Scholar
  10. Herrmann RF& Shann JF (1997) Microbial community changes during the composting of municipal solid waste. Microb. Ecol. 33: 78-85Google Scholar
  11. Inbar Y, Chen Y, Hadar Y& Verdonck O (1988) Composting of agricultural wastes for their use as container media: simulation of the composting process. Biol. Wastes 26: 241-259Google Scholar
  12. Inbar Y, Hadar Y& Chen Y (1993) Recycling of cattle manure: the composting process and characterization of maturity. J. Environ. Qual. 22: 857-863Google Scholar
  13. Insam H, Amor K, Renner M& Crepaz C (1996) Changes in functional abilities of the microbial community during composting of manure. Microb. Ecol. 31: 77-87Google Scholar
  14. Itävaara M, Venelampi O, Vikman M& Kapanen A (2002) Compost maturity-problems associated with testing. Proceedings Microbiology of Composting, Innsbruck, Austria 18.-21.10.2000 (pp 373-383). Springer Verlag, HeidelbergGoogle Scholar
  15. Jenkinson DS (1971) Studies on the decomposition of C14 labelled organic matter in soil. Soil Sci. 111: 64-70Google Scholar
  16. Klamer M& Bååth E (1998) Microbial community dynamics during composting of straw material using phospholipid fatty acid analysis. FEMS Microbiol. Ecol. 27: 9-20Google Scholar
  17. Kuzyakov Y, Friedel JK& Stahr K (2000) Review of mechanisms and quantification of priming effects. Soil Biol. Biochem. 32: 1485-1498Google Scholar
  18. Maheshwari R, Bharadwaj G& Bhat MK (2000) Thermophilic fungi: their physiology and enzymes. Microbiol. Molecular Biol. Rev. 64: 461-488Google Scholar
  19. Martin JP& Haider K (1979) Effect of concentration on decomposition of some 14C-labeled phenolic compounds, benzoic acid, glucose, cellulose, wheat straw, and Chlorella protein in soil. Soil Sci. Soc. Am. J. 43: 917-920Google Scholar
  20. Martin JP, Haider K, Farmer WJ& Fustec-Mathon E (1974) Decomposition and distribution of residual activity of some 14C-microbial polysaccharides and cells, glucose, cellulose and wheat straw in soil. Soil Biol. Biochem. 6: 221-230Google Scholar
  21. Martin JP, Parsa AA& Haider K (1978) Influence of intimate association with humic polymers on biodegradation of [14C]labeled organic substrates in soil. Soil Biol. Biochem. 10: 483-486Google Scholar
  22. Pagga U (1999) Compostable packaging materials-test methods and limit values for biodegradation. Appl.Microbiol. Biotechnol. 51: 125-133Google Scholar
  23. Senesi N (1989) Composted materials as organic fertilizers. Sci. Total Environ. 81/82: 521-542Google Scholar
  24. Sharabi, NE-D& Bartha R (1993) Testing of some assumptions about biodegradability in soil as measured by carbon dioxide evolution. Appl. Environ. Microbiol. 59: 1201-1205Google Scholar
  25. Shen J& Bartha R (1996) Priming effect of substrate addition in soil-based biodegradation tests. Appl. Environ. Microbiol. 62: 1428-1430Google Scholar
  26. Shen J& Bartha R (1997) Priming effect of glucose polymers in soil-based biodegradation tests. Soil Biol. Biochem. 29: 1195-1198Google Scholar
  27. Stewart BJ& Leatherwood JM (1976) Derepressed synthesis of cellulase by Cellulomonas. J. Bacteriol. 128: 609-615Google Scholar
  28. Sørensen LH (1974) Rate of decomposition of organic matter in soil as influenced by repeated air drying-rewetting and repeated additions of organic material. Soil Biol. Biochem. 6: 287-292Google Scholar
  29. Tate RL (1987) Soil Organic Matter. Biological and Ecological Effects. John Wiley&Sons, USAGoogle Scholar
  30. Tuomela M, Lyytikäinen M, Oivanen P& Hatakka A (1999) Mineralization and conversion of pentachlorophenol (PCP) in soil inoculated with the white-rot fungus Trametes versicolor. Soil Biol. Biochem. 31: 65-74Google Scholar
  31. TuomelaM, VikmanM, Hatakka A& Itävaara M(2000) Biodegradation of lignin in a compost environment: a review. Bioresource Technol. 72: 169-183Google Scholar
  32. TuomelaM, Hatakka A, Raiskila S, Vikman M& Itävaara M(2001) Biodegradation of radiolabelled synthetic lignin (14C-DHP) and mechanical pulp in a compost environment. Appl. Microbiol. Biotechnol. 55: 492-499Google Scholar
  33. Veeken A, Nierop K, de Wilde V& Hamlers B (2000) Characterization of NaOH-extracted humic acids during composting of a biowaste. Bioresource Technol. 72: 33-41Google Scholar
  34. Wu J, Brookes PC& Jenkinson DS (1993) Formation and destruction of microbial biomass during the decomposition of glucose and ryegrass in soil. Soil Biol. Biochem. 25: 1435-1441Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Marja Tuomela
  • Annele Hatakka
  • Sari Karjomaa
  • Merja Itävaara

There are no affiliations available

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