Plant and Soil

, Volume 245, Issue 2, pp 215–222 | Cite as

The effect of inoculation with Azospirillum brasilense on growth and nitrogen utilization by wheat plants

  • María I. Saubidet
  • Nora Fatta
  • Atilio J. Barneix

Abstract

Azospirillium brasilense is a rhizosphere bacteria that has been reported to improve yield when inoculated on wheat plants. However, the mechanisms through which this effect is induced is still unclear. In the present work, we have studied the effects of inoculating a highly efficient A. brasilense strain on wheat plant grown in 5 kg pots with soil in a greenhouse, under three N regimes (0, 3 or 16 mM NO3, 50 ml/pot once or twice-a -week), and in disinfected or non-disinfected soil. At the booting stage, the inoculated roots in both soils showed a similar colonization by Azospirillum sp. that was not affected by N addition. The plants grown in the disinfected soil showed a higher biomass, N content and N concentration than those in the non-disinfected soil, and in both soils the inoculation stimulated plant growth, N accumulation, and N and NO3 concentration in the tissues.

At maturity, the inoculated plants showed a higher biomass, grain yield and N content than the uninoculated ones in both soils, and a higher grain protein concentration than the uninoculated. It is concluded that in the present experiments, A. brasilenseincreased plant growth by stimulating nitrogen uptake by the roots.

Azospirillum brasilense inoculation nitrogen uptake Triticum aestivum

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References

  1. Alexander M 1965 Most-probable-number method for microbial population. In Methods of Soil Analysis. Part II. Ed. C A Black, pp 1467-1471. American Society of Agronomy, Madison, WI. USA.Google Scholar
  2. Bashan Y, Harrison S K and Whitmoyer RE 1990 Enhanced growth of wheat and soybean plants inoculated with Azospirillum brasilense is not necessarily due to general enhancement of mineral uptake. App. Environ. Microbiol. 56, 769-775.Google Scholar
  3. Bashan Y 1999 Interactions of Azospirillum spp. in soils: A review. Biol. Fert. Soils. 29, 246-256.Google Scholar
  4. Bashan Y and Holguin G 1997 Azospirillum-plant relationships: environmental and physiological advances (1990-1996). Can. J. Microbiol. 43, 103-121.Google Scholar
  5. Boddey R M, Baldani V L D, Baldani J I and Döbereiner J 1986 Effect of inoculation of Azospirillum spp. on nitrogen accumulation by field-grown wheat. Plant Soil 95, 109-121.Google Scholar
  6. Cattaldo D A, Haroon M, Schrader L E and Young V L 1975 Rapid colorimetric determination of nitrate in plant tissue by nitration of salycilic acid. Commun. Soil Sci. and Plant Ann. 6, 71-80.Google Scholar
  7. Crawford N M and Glass A D M 1998 Molecular and physiological aspects of nitrate uptake in plants. Trends Plant Sci. 3, 389-395.Google Scholar
  8. Döbereiner J 1992 History and new perspectives of diazotrophs in association with non-leguminous plants. Symbiosis 13, 1-13.Google Scholar
  9. Döbereiner J and Baldani V L D 1979 Selective infection of maize roots by streptomycin-resistant Azospirillum lipoferum and other bacteria. Can. J. Microbiol. 25, 1264-1269.Google Scholar
  10. Guitman M R, Arnozis P A and Barneix A J 1991 Effect of source-sink relations and nitrogen nutrition on senescence and N remobilization in the flag leaf of wheat. Physiol. Plant. 82, 278-284.Google Scholar
  11. Hänisch ten Cate C H and Breteler H 1982 Effects of plant growth regulators on nitrate utilization by roots of nitrogen depleted dwarf bean. J. Exp. Bot. 33, 37-46.Google Scholar
  12. Hoagland D R and Arnon D I 1950 The Water Cultured Method for Growing PlantsWithout Soil. California Agric. Exp. St., Circular 347. Oakland. C.A. USA.Google Scholar
  13. Imsande J and Touraine B 1994 N demand and the regulation of nitrate uptake. Plant Physiol. 105, 3-7.Google Scholar
  14. Jagnow G 1990 Differences between cereal crop cultivars in rootassociated nitrogen fixation, possible causes of variable yield response to seed inoculation. Plant Soil 123, 255-259.Google Scholar
  15. Kapulnik Y, Gafni R. and Okon Y 1985 Effect of Azospirillum spp. inoculation on root development and NO3-uptake in wheat (Triticum aestivum cv.Miriam) in hydroponic system. Can. J. Bot. 63, 187-196.Google Scholar
  16. Krieg N R 1984 Aerobic/microarophilic, motile, helical/vibroid Gram-negative bacteria. In Bergey's Manual of Systematic Bacteriology. Vol. 1. Eds. NR Krieg and JG Holt, pp. 71-124. Williams and Willkins, Baltimore/London.Google Scholar
  17. Mertens T and Hess D 1984 Yield increases in spring wheat (Triticum aestivum L.) inoculated with Azospirillum lipoferum under greenhouse and field conditions of a temperate region. Plant Soil 82, 87-99.Google Scholar
  18. Millet E, Avivi Y and Feldman M 1985 Effects of rhizosferic bacteria on wheat yield under field conditions. Plant Soil 86, 347-355.Google Scholar
  19. New P B and Kennedy I R 1989 Regional distribution and pH sensitivity of Azospirillum associated with wheat roots in eastern Australia. Microbiol. Ecol. 17, 299-309.Google Scholar
  20. Okon Y and Kapulnik Y 1986 Development and function of Azospirillum-inoculated roots. Plant Soil. 90, 3-16.Google Scholar
  21. Okon Y and Lavandera-Gonzalez C A 1994 Agronomic applications of Azospirillum: An evaluation of 20 years worldwide field inoculation. Soil Biol. Biochem. 26, 1591-1601.Google Scholar
  22. Paul E A and Clark F E 1989 Soil microbiology and biochemistry. Academic Press, San Diego, Ca., USA. 275 pp.Google Scholar
  23. Rai, S N and Gaur C 1982 Nitrogen fixation by Azospirillum spp and effect of Azospirillum lipoferum on the yield and N-uptake of wheat crop. Plant Soil 69, 233-238.Google Scholar
  24. Reynders L and Vlassak K 1982 Use of Azospirillum brasilense as biofertilizer in intensive wheat cropping. Plant Soil 66, 217-273.Google Scholar
  25. Saubidet M I and Barneix A J 1998 Growth Stimulation and Nitrogen Supply to Wheat Plants Inoculated with Azospirillum brasilense. J. Plant Nut. 21, 2565-2577.Google Scholar
  26. Simpson R J, Lambers H and Dalling M J 1982 Kinetin application to roots and its effect on uptake, translocation and distribution of nitrogen in wheat (Triticum aestivum) grown with a split root system. Physiol Plant. 56, 430-435.Google Scholar
  27. Sood C R, Chanda S V and Singh Y D 1995 Effect of plant growth regulators on nitrate uptake and its reduction in radish cotyledons. J. Plant Nut. 18, 2595-2607.Google Scholar
  28. Spiertz J H J and de Vos N M 1983 Agronomic and physiological aspects of the role of nitrogen in yield formation of cereals. Plant Soil 75, 379-392.Google Scholar
  29. Vande Broek A, Michiellis J, Van Gool A and Vanderleyden J 1993 Spatial-temporal colonization patterns of Azospirillum brasilense on the wheat root surface and expression of the bacteria nifH gene during association. Molec. Plant-Microbe Interac. 6, 5921-6000.Google Scholar
  30. Wagner B M and Beck E 1993 Cytokinins in the perennial herb Urtica dioica L. as influenced by its nitrogen status. Planta 190, 511-518.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • María I. Saubidet
    • 1
  • Nora Fatta
    • 1
  • Atilio J. Barneix
    • 1
  1. 1.CONICET- Facultad de Agronomía U.B.A.IBYFBuenos AiresArgentina

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