Advertisement

Randomized Autocontrolled Comparison of the Embryo Culture Performance of Nunc and Falcon Petri Dishes

  • M. Van Den Bergh
  • S. Emiliani
  • J. Biramane
  • A.-S. Vannin
  • Y. Englert
Article

Abstract

Purpose: Our purpose was to compare the embryo culture performance of two types of petri dishes (Nunc and Falcon).

Methods: Mouse zygotes were cultured up to the expanded blastocyst stage in both types of dishes. The oocytes from 50 in vitro fertilization cycles were randomly divided between the two types of dishes. Fertilization, cleavage, and embryo quality were compared. Oocytes from another 50 cycles were all cultured at random in either type of dish. Pregnancy and implantation rates were compared between the two types.

Results: Of 91 mouse zygotes, 81 cleaved to two-cell-stage embryos, and 64 became expanded blastocysts in Falcon dishes; of 99 zygotes, 81 cleaved to two-cell-stage embryos and 66 became expanded blastocysts in Nunc dishes. Of 248 oocyte–cumulus complexes (OCC), 145 fertilized in Falcon dishes, and of 269 OCC, 175 fertilized in Nunc dishes. The high quality embryo ratio was 51 out of 118 in Falcon dishes, not different from that in Nunc dishes, 58 out of 139. In Falcon dishes 72 out of 118 embryos were at least at the four-cell stage after 45 hr, versus 70 out of 139 in Nunc dishes. Twenty-three clinical pregnancies were obtained in the first 50 cycles with sibling oocytes. In the second group, with randomization of the cycles between Nunc and Falcon, 8 pregnancies were obtained in the Nunc and 10 in the Falcon dishes. The implantation rate in this second group of 50 cycles was 9 out of 61 in Falcon and 11 out of 57 in Nunc dishes.

Conclusions: No differences were observed.

petri dishes quality control embryo toxicity in vitro fertilization 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

REFERENCES

  1. 1.
    Edwards RG, Purdy JM, Steptoe PC, Walters DE: The growth of human preimplantation embryos in vitro. Am J Obstet Gynecol 1981;141:408-416Google Scholar
  2. 2.
    Fishel SB, Edwards RG, Evans CJ: Human chorionic gonadotropin secreted by preimplantation embryos cultured in vitro. Science 1984;223:816-818Google Scholar
  3. 3.
    Lopata A, Hay DL: The potential of early human embryos to form blastocysts, hatch from their zona and secrete hCG in culture. Hum Reprod 1989;4(Suppl): 87-94Google Scholar
  4. 4.
    Bolton VN, Hawes SM, Taylor TC, Parson JH: Development of spare human preimplantation embryos in vitro: An analysis of the correlations among gross morphology, cleavage rates, and the development to the blastocyst. J Vitro Fertil Embryo Transfer 1989;6:30-35Google Scholar
  5. 5.
    Muggleton-Haris A, Findlay I, Whittingham DG: Improvement of the culture conditions for the development of human preimplantation embryos. Hum Reprod 1990;5:217-220Google Scholar
  6. 6.
    Bavister BD: Culture of preimplantation embryos: Facts and artefacts. Hum Reprod Update 1995;1/2:91-148Google Scholar
  7. 7.
    de Haan RL: Toxicity of tissue culture media exposed to polivinyl chloride plastic. Nature (New Biol) 1971;231:85-86Google Scholar
  8. 8.
    Van den Bergh M, Baszo I, Bertrand E, Biramane J, Englert Y: Quality control in IVF with mouse bioassays: Four years' experience. J Assist Reprod Genet 1996;13:739-744Google Scholar
  9. 9.
    Devreker F, Govaerts I, Bertrand E, Van den Bergh M, Gervy C, Englert Y: The long-acting gonaditropin-releasing hormone analogues impaired the implantation rate. Fertil Steril 1996;65(1):122-126Google Scholar
  10. 10.
    Tea NT: A migration gravidity sedimentation method for collecting motile spermatozoa from human semen. In In Vitro Fertilization, Embryo Transfer and Early Pregnancy, RF Harrison, J Bonnar, W Thompson (eds). Lancaster, Boston, The Hague, Dordrecht, MTP Press, 1983Google Scholar
  11. 11.
    Puissant F, Van Rysselberge M, Barlow P, Deweze J, Leroy F: Embryo scoring as a prognostic tool in IVF treatment. Hum Reprod 1987;2:705-708Google Scholar

Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • M. Van Den Bergh
    • 1
  • S. Emiliani
    • 1
  • J. Biramane
    • 1
  • A.-S. Vannin
    • 1
  • Y. Englert
    • 1
  1. 1.Fertility Clinic, Hôpital ErasmeFrench Speaking Free University BrusselsBrusselsBelgium

Personalised recommendations