Clinical & Experimental Metastasis

, Volume 19, Issue 6, pp 477–486

Establishment of human osteosarcoma cell lines with high metastatic potential to lungs and their utilities for therapeutic studies on metastatic osteosarcoma

  • Kenji Kimura
  • Tetsuhiro Nakano
  • Yong-Bum Park
  • Masachika Tani
  • Hiroyuki Tsuda
  • Yasuo Beppu
  • Hideshige Moriya
  • Jun Yokota


Relevant animal models for metastasis of osteosarcoma is needed to understand the biology and to develop the treatment modality of metastasis of human osteosarcoma. Therefore, we screened six human osteosarcoma cell lines for metastatic ability in nude mice. The HuO9 cell line was identified as being metastatic to the lung after intravenous injection. We established two sublines, HuO9-M112 and HuO9-M132, with high metastatic potential to the lung from the parental HuO9 cells by in vivo selection. There were no differences between these two sublines and the parental cells in the growth rate in vitro and the tumorigenicity after subcutaneous injection in nude mice, however, mice injected with the metastatic sublines became moribund earlier than mice injected with the parental HuO9 cells did. Thus, adriamycin (ADR) and recombinant interleukin-12 (IL-12) were administered to mice injected with the HuO9-M112 subline to suppress experimental lung metastases. Production of lung colonies was significantly suppressed and the prognoses of mice were significantly improved by both ADR and IL-12 treatments. These results indicate that both ADR and IL-12 are effective agents against pulmonary metastatic osteosarcoma, and that these sublines are useful for studies on the biological behavior and treatment of pulmonary metastatic osteosarcoma.

adriamycin human osteosarcoma cell line in vivo selection interleukin-12 lung metastasis 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Kenji Kimura
    • 1
    • 2
  • Tetsuhiro Nakano
    • 1
  • Yong-Bum Park
    • 1
  • Masachika Tani
    • 1
  • Hiroyuki Tsuda
    • 3
  • Yasuo Beppu
    • 4
  • Hideshige Moriya
    • 2
  • Jun Yokota
    • 1
  1. 1.Biology and Chemotherapy DivisionsNational Cancer Center Research InstituteTokyoJapan
  2. 2.Department of Orthopedic SurgeryChiba UniversityChibaJapan
  3. 3.Experimental Pathology and Chemotherapy DivisionsNational Cancer Center Research InstituteTokyoJapan
  4. 4.Orthopedic DivisionNational Cancer Center HospitalTokyoJapan
  5. 5.Biology DivisionNational Cancer Center Research InstituteTokyoJapan

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