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Molecular Breeding

, Volume 10, Issue 1–2, pp 71–82 | Cite as

Wheat LEA genes, PMA80 and PMA1959, enhance dehydration tolerance of transgenic rice (Oryza sativa L.)

  • Zaiquan Cheng
  • Jayaprakash Targolli
  • Xingqi Huang
  • Ray Wu
Article

Abstract

Drought and salt stresses are two major factors that lower plant productivity. Transgenic approaches offer powerful means to better understand and then minimize loss of yield due to these abiotic stresses. In this study, we have generated transgenic rice plants expressing a wheat LEA group 2 protein (PMA80) gene, and separately the wheat LEA group 1 protein (PMA1959) gene. Molecular analysis of the transgenic plants revealed the stable integration of the transgenes. Immunoblot analysis showed the presence of the LEA group 2 protein (39 kDa) and the LEA group 1 protein (25 kDa) in most of the plant lines. Second-generation transgenic plants were subjected to dehydration or salt stress. The results showed that accumulation of either PMA80 or PMA1959 correlates with increased tolerance of transgenic rice plants to these stresses.

Dehydration tolerance Transgenic rice Wheat LEA genes 

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References

  1. Allen G.C., Hall G.P., Michalowski S., Newman W., Spiker S., Weissinger A.K. et al. 1996. High-level transgene expression in plant cells: effects of a strong scaffold attachment region from tobacco. Plant Cell 8: 899-913.CrossRefPubMedGoogle Scholar
  2. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A. et al. 1994. Current Protocols in Molecular Biology. John Wiley & Sons Inc, USA.Google Scholar
  3. Baker J., Steele C. and Dure L. III 1988. Sequence and characteristics of six LEA proteins and their genes from cotton. Plant Mol. Biol. 11: 277-291.Google Scholar
  4. Bajaj S., Targolli J., Liu L.F., Ho T.-H.D. and Wu R. 1999. Transgenic approaches to increase dehydration-stress tolerance in plants. Mol. Breed 5: 493-503.Google Scholar
  5. Bohnert H.J. and Jensen R.G. 1996. Strategies for engineering water stress tolerance in plants. Trends in Biochem. 14: 89-97.Google Scholar
  6. Boyer J.S. 1982. Plant productivity and environment. Science 218: 443-448.Google Scholar
  7. Bradford M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.CrossRefPubMedGoogle Scholar
  8. Cadle M.M., Rayfuse L.M., Walker-Simmons M.K. and Jones S.S. 1994. Mapping of abscisic acid responsive genes and vp1 to chromosomes in wheat and Lophopyrum elongatum. Genome. 37: 129-132.Google Scholar
  9. Cao J., Duan X., McElroy D. and Wu R. 1992. Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. Plant Cell Rep. 11: 586-591.CrossRefGoogle Scholar
  10. Chandler P.M. and Robertson M. 1994. Gene expression regulated by abscisic acid and its relation to stress tolerance. In: Somerville C.R. and Jones R.L. (eds), Annual Review of Plant Physiology and Plant Molecular Biology. Annual Reviews Inc., pp. 113-141.Google Scholar
  11. Duan X., Li X., Xue Q., Abo-El-Saad M., Xu D. and Wu R. 1996. Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistant. Nature Biotech. 14: 494-498.Google Scholar
  12. Dure L. III, Crouch M., Harada J., Ho T.-H.D., Mundy J., Quatrano R.S. et al. 1989. Common amino acid sequence domains among the LEA proteins of higher plants. Plant Mol. Biol. 12: 475-486.Google Scholar
  13. Dure L. III 1992. The LEA proteins of higher plants. In: Verma D.P.S. (ed.), Control of Plant Gene Expression. CRC Press, Boca Raton, Florida, USA, pp. 325-335.Google Scholar
  14. Dure L. III 1993. A repeating 11-mer amino acid motif and plant desiccation. Plant J. 3: 363-369.PubMedGoogle Scholar
  15. Finnegan J. and McElroy D. 1994. Transgene inactivation: plants fight back!. Bio/Technology 12: 883-888.CrossRefGoogle Scholar
  16. Hiei Y., Ohta S., Komari T. and Kumashiro T. 1994. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of boundaries of T-DNA. Plant J. 6: 271-282.CrossRefPubMedGoogle Scholar
  17. Holmberg N. and Bulow N. 1998. Improving stress tolerance in plants by gene transfer. Trends Plant Sci. 3: 61-66.Google Scholar
  18. Hong B., Uknes S.J. and Ho T.-H.D. 1988. Cloning and characterization of a cDNA encoding an mRNA rapidly induced by ABA in barley aleurone layers. Plant Mol. Biol. 11: 495-506.Google Scholar
  19. Jayaprakash T.L., Ramamohan G., Krishnaprasad B.T., Ganeshkumar, Prasad T.G., Mathew M.K. et al. 1998. Genotypic variabilities in differential expression of lea 2 and lea 3 genes and proteins in response to salinity stress in finger millet (Eleusine coracana Gaertn) and rice (Oryza sativa L.) seedlings. Annals of Botany (London) 82: 513-522.Google Scholar
  20. Jones H., Leigh R.A., Tomos A.D. and Jones R.G.E. 1987. The effect of abscisic acid on cell turgor pressure, solute content and growth of wheat roots. Planta. 170: 257-262.Google Scholar
  21. Kumpatla S.P., Chandrasekharan M.B., Iyer L.M., Li G. and Hall T.C. 1998. Genome intruder scanning and modulation systems and transgene silencing. Trends Plant Sci. 3: 97-104.Google Scholar
  22. McElroy D., Blowers A.D., Jenes B. and Wu R. 1991. Construction of expression vectors based on the rice actin1 (Act1) 5' region for use in monocot transformation. Mol. Gen. Genet. 231: 150-160.PubMedGoogle Scholar
  23. Moons A., Bauw G., Prinsen E., Van Montagu M. and Straeten D.V.D. 1995. Molecular and physiological responses to abscisic acid and salts in roots of salt sensitive and salt tolerant Indica rice varieties. Plant Physiol. 107: 177-186.PubMedGoogle Scholar
  24. Morris C.F., Andererberg R.J., Goldmark P.J. and Walker-Simmons M.K. 1991. Molecular cloning and expression of abscisic acid responsive genes in embryos of dormant wheat seeds. Plant Physiol. 95: 814-821.Google Scholar
  25. Mundy J. and Chua N.H. 1988. Abscisic acid and water stress induce the expression of a novel rice gene. EMBO J. 7: 2279-2286.PubMedGoogle Scholar
  26. Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-479.Google Scholar
  27. Reid J.B. 1993. Plant hormone mutants. J. Plant Growth Reg. 12: 207-226.Google Scholar
  28. Sheppard L.J., Franssen I. and Cape J.N. 1995. Frost hardiness of Norway spruce treated with acid mist. Evolution of the electrolyte leakage rate technique. Environ. and Experimen. Bot. 35: 139-149.Google Scholar
  29. Sokal R.R. and Rohlf F.J. 1969. Biometry: The Principles and Practice of Statistics in Biological Research. W.F. Freeman, San Francisco.Google Scholar
  30. Stacy R.A.P., Espelund M., Saeboe L.S., Hollun K. and Jakobsen K.S. 1995. Evolution of the group 1 late embryogenesis abundant (LEA) genes: analysis of the LEA B19 gene family in barley. Plant Mol. Biol. 28: 1039-1054.PubMedGoogle Scholar
  31. Stam M., Mol J.N.M. and Kooter J.M. 1997. The silence of genes in transgenic plants. Annals of Botany 79: 3-12.Google Scholar
  32. Tarczynski M.C., Jensen R.G. and Bohnert H.J. 1993. Stress protection of transgenic tobacco by production of the osmolyte mannitol. Science 259: 508-510.Google Scholar
  33. Vam P., Worland B., Kohli A., Snape J.W., Christou P., Allen G.C. et al. 1999. Matrix attachment regions increase transgene expression levels and stability in transgenic rice plants and their progeny. Plant J. 18: 233-242.Google Scholar
  34. Wang B. and Wu R. 1995. A vector for inserting foreign genes and selection of transformed rice plants. Rice Biotech. Quart. 22: 8.Google Scholar
  35. Xu D., Duan X., Wang B., Hong B., Ho T.-H.D. and Wu R. 1996. Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol. 110: 249-257.PubMedGoogle Scholar
  36. Zhao X., Wu T., Xie Y. and Wu R. 1989. Genome specific repetitive sequence in the genus Oryza. Theor. Appl. Genet. 78: 201-209.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Zaiquan Cheng
    • 1
  • Jayaprakash Targolli
    • 1
  • Xingqi Huang
    • 1
  • Ray Wu
    • 1
  1. 1.Department of Molecular Biology and GeneticsCornell UniversityIthacaUSA

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