Molecular Biology Reports

, Volume 29, Issue 1–2, pp 83–87 | Cite as

Biphasic Oxygen Kinetics of Cellular Respiration and Linear Oxygen Dependence of Antimycin A Inhibited Oxygen Consumption

  • Eveline Hütter
  • Kathrin Renner
  • Pidder Jansen-Dürr
  • Erich Gnaiger
Article

Abstract

Oxygen kinetics in fibroblasts was biphasic. This was quantitatively explained by a major mitochondrial hyperbolic component in the low-oxygen range and a linear increase of rotenone-and antimycin A-inhibited oxygen consumption in the high-oxygen range. This suggests an increased production of reactive oxygen species and oxidative stress at elevated, air-level oxygen concentrations. The high oxygen affinity of mitochondrial respiration provides the basis for the maintenance of a high aerobic scope at physiological low-oxygen levels, whereas further pronounced depression of oxygen pressure induces energetic stress under hypoxia.

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Eveline Hütter
    • 1
  • Kathrin Renner
    • 2
  • Pidder Jansen-Dürr
    • 1
  • Erich Gnaiger
    • 3
  1. 1.Institute for Biomedical Aging ResearchAustrian Academy of SciencesInnsbruckAustria
  2. 2.Tyrolean Cancer Research InstituteInnsbruckAustria
  3. 3.Department of Transplant Surgery, D. Swarovski Research LaboratoryUniversity Hospital InnsbruckInnsbruck

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