Molecular Biology Reports

, Volume 29, Issue 1–2, pp 157–161 | Cite as

Time Dependent Responses of Glycolytic Intermediates in a Detailed Glycolytic Model of Lactococcus Lactis During Glucose Run-Out Experiments

  • M.H.N. Hoefnagel
  • A. van der Burgt
  • D.E. Martens
  • J. Hugenholtz
  • J.L. Snoep
Article

Abstract

Glucose addition and subsequent run-out experiments were compared to simulations with a detailed glycolytic model of Lactococcus lactis. The model was constructed largely on bases of enzyme kinetic data taken from literature and not adjusted for the specific simulations shown here. Upon glucose depletion a rapid increase in PEP, inorganic phosphate and a gradual decrease in fructose 1,6-bisphosphate (FBP) were measured and predicted by simulation. The dynamic changes in these and other intermediate concentrations as measured in the experiments were well predicted by the kinetic model.

metabolic engineering Lactococcus lactis kinetic modelling 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Fordyce, A. M., Crow, V. L. and Thomas, T. D. 1984. Appl. Env. Microbiol. 48, 332–337.Google Scholar
  2. Hoefnagel, M. H. N., Starrenburg, M. J. C., Martens, D. E., Hugenholtz, J., Kleerebezem, M., Van Swam, I. I., Bongers, R., Westerhoff, H. V., Snoep, J. L. 2002. Microbiology 148, 1003–1013.Google Scholar
  3. Mendes, P. 1993. Comp. Appl. Biosci. 9, 563–571.Google Scholar
  4. Neves, A. R. Ramos, A., Shearman, C., Gasson, M. J., Almeida, J. S. and Santos, H. 1999. Biotech. Bioeng. 64, 200–212.Google Scholar
  5. Novak, L. and Loubiere, P. 2000. J Bacteriol 182, 1136–1143.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • M.H.N. Hoefnagel
    • 1
  • A. van der Burgt
    • 1
  • D.E. Martens
    • 1
  • J. Hugenholtz
    • 2
  • J.L. Snoep
    • 3
    • 4
  1. 1.Wageningen Centre for Food Sciences, Food and Bioprocess Engineering GroupWageningen UniversityWageningenThe Netherlands
  2. 2.NIZO Food ResearchEdeThe Netherlands
  3. 3.Dept. of BiochemistryUniversity of StellenboschMatielandSouth Africa
  4. 4.Molecular Cell PhysiologyVrije UniversiteitAmsterdamThe Netherlands

Personalised recommendations