Plant and Soil

, Volume 244, Issue 1–2, pp 19–28 | Cite as

Fungal diversity in ectomycorrhizal communities: sampling effort and species detection

  • Andy F. S. Taylor


A number of recent review articles on ectomycorrhizal (ECM) fungal community diversity have highlighted the unprecedented increase in the number of publications on this ecologically important but neglected area. The general features of these species-rich, highly dynamic and complex communities have been comprehensively covered but one aspect crucial to our assessment of diversity, namely the sampling of ECM communities has received less attention. This is a complex issue with two principal components, the physical sampling strategy employed and the life cycle traits of the ECM fungi being examined. Combined, these two components provide the image that we perceive as ECM diversity. This contribution will focus primarily on the former of these components using a recent study from a pine forest in central Sweden to highlight some sampling problems and also to discuss some features common to ECM communities. The two commonly used elements of diversity, species richness and community evenness, present rather different problems in the assessment of ECM diversity. The applicability of using current measures of abundance (number or percentage of root tips colonised) to determine community evenness is discussed in relation to our lack of knowledge on the size of individual genets of ECM fungi. The inherent structure of most ECM communities, with a few common species and a large number of rare species, severely limits our ability to accurately assess species richness. A discussion of theoretical detection limits is included that demonstrates the importance of the sampling effort (no. of samples or tips) involved in assessing species richness. Species area abundance plots are also discussed in this context. It is suggested that sampling strategy (bulk samples versus multiple collections of single tips) may have important consequences when sampling from communities where root tip densities differ. Finally, the need for studies of the spatial distribution of ECM on roots in relation to small-scale soil heterogeneity and of the temporal aspects of ECM community dynamics is raised.

community structure ectomycorrhizas fungal distribution species richness vertical distribution 


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  1. Agerer R 1986-1998 Colour Atlas of Ectomycorrhizae. Schwäbisch-Gmünd, Einhorn-Verlag.Google Scholar
  2. Anderson I C, Chambers S M, Cairney J W G 1998 Use of molecular methods to estimate the size and distribution of mycelial individuals of the ectomycorrhizal basidiomycete Pisolithus tinctorius. Mycol. Res. 102, 295-300.Google Scholar
  3. Baar J, Ozinga W A and Kuyper Th W 1994 Spatial distribution of Laccaria bicolor genets reflected by sporocarps after removal of litter and humus layers in a Pinus sylvestris forest. Mycol. Res. 98(7), 726-728.Google Scholar
  4. Baar J, Horton T R, Kretzer A M and Bruns T D 1999 Mycorrhizal colonization of Pinus muricata from resistant propagules after a stand replacing wildfire. New Phytol. 143, 409-418.Google Scholar
  5. Bamforth S S 1995 Interpreting soil ciliate biodiversity. Plant Soil 170, 159-164.Google Scholar
  6. Bebber D, 1999 Spatial autocorrelations. TREE 14(5), 196.Google Scholar
  7. Bringmark E and Bringmark L 1998 Improved soil monitoring by use of spatial patterns. Ambio 27, 45-52.Google Scholar
  8. Bruns T D 1995 Thoughts on the processes that maintain local species diversity of ectomycorrhizal fungi. Plant Soil 170, 63-73.Google Scholar
  9. Copley J 2000 Ecology goes underground. Nature 406, 452-454.Google Scholar
  10. Dahlberg A 2001 Community ecology of ectomycorrhizal fungi: an advancing interdisciplinary field. New Phytol. 150, 555-562.Google Scholar
  11. Dahlberg A and Stenlid J 1990 Size, distribution and biomass of genets in populations of Suillus bovinus (L.:Fr.) Ruossel revealed by somatic incompatibility. New Phytol. 115, 487-493.Google Scholar
  12. Dahlberg A, Jonsson L and Nylund J-E 1997 Species diversity and distribution of biomass above-and below-ground among ectomycorrhizal fungi in an old-growth Norway spruce forest in south Sweden. Can. J. Bot. 75, 1323-1335.Google Scholar
  13. Danielson R M and Visser S 1989 Effects of forest soil acidification on ectomycorrhizal and vesicular-arbuscular mycorrhizal development. New Phytol. 112: 41-48Google Scholar
  14. Dushyantha K, Hutchings W and Hutchings M J 1997 The effects of spatial scale of environmental heterogeneity on the growth of a clonal plant: an experimental study with Glechoma hederacea. J. Ecol. 85, 17-28.Google Scholar
  15. Erland S and Taylor A F S 2002 Diversity of ectomycorrhizal communities in relation to the abiotic environment. In Mycorrhizal Ecology. Eds. Marcel G A van der Heijden &; Ian R Sanders. pp 470. Ecological Studies Series. Vol 157. ch 7. Springer-Verlag. Berlin.Google Scholar
  16. Fransson P M A, Taylor A F S, Finlay R D 2000 Effects of optimal fertilization on belowground ectomycorrhizal community structure in a Norway spruce forest. Tree Phys. 20, 599-606Google Scholar
  17. Gardes M and Bruns T D 1996 Community structure of ectomycorrhizal fungi in a Pinus muricata forest: above-and below-ground views. Can. J. Bot. 74, 1572-1583Google Scholar
  18. Gherbi H, Delaruelle C, Selosse M-A and Martin F 1999 High genetic diversity in a population of the ectomycorrhizal basidiomycete Laccaria amethystina in a 150-year-old beech forest. Mol. Ecol. 8, 2003-2013.Google Scholar
  19. Giller P S 1996 The diversity of soil communities, the 'poor man's tropical rainforest'. Biol. Con. 5, 135-168.Google Scholar
  20. Grundmann G L and Debouzie D 2000 Geostatistical analysis of the distribution of NH4 + and NO2 ?-oxidizing bacteria and serotypes at the millimetre scale along a soil transect. FEMS Microbiol. Ecol. 34, 57-62Google Scholar
  21. Hansen P A 1988 Prediction of macrofungal occurrence in Swedish beech forest from soil and litter variable models. Vegetatio 78, 31-44.Google Scholar
  22. Harvey A E, Larsen M J and Jurgensen M F 1976 Distribution of ectomycorrhizae in a mature Douglas fir larch forest soil inWestern Montana. For. Sci. 22(4), 393-398.Google Scholar
  23. Harvey A E, Larsen M J and Jurgensen M F 1978 Comparative distribution of ectomycorrhizae in soils of three Western Montana forest habitat types. For. Sci. 25(2), 350-358.Google Scholar
  24. Heath D 1995 An Introduction to Experimental Design and Statistics for Biology. UCL Press, University College London, UK. 372.Google Scholar
  25. Horton T R and Bruns T D 2001 The molecular revolution in ectomycorrhizal ecology: peeking into the black box. Mol. Ecol. 10, 1855-1871.Google Scholar
  26. Jackson R B and Caldwell M M 1993 Geostatistical patterns of soil heterogeneity around individual perennial plants. J. Ecol. 81, 683-692.Google Scholar
  27. Krebs C J 1989 Ecological methodology, 2nd ed. Harper &; Row, New York. Kreutzer K 1995 Effects of forest liming on soil processes. Plant Soil 168-169, 447-470.Google Scholar
  28. Kropp B R 1982 Formation of mycorrhizae on nonmycorrhizal Western Hemlock outplanted on rotten wood and mineral soil. For. Sci. 28(4), 706-710.Google Scholar
  29. Kårén O, Hogberg N, Dahlberg A, Jonsson L and Nylund J-E 1997 Inter-and intraspecific variation in the ITS region of rDNA of ectomycorrhizal fungi in Fennoscandia as detected by endonuclease analysis. New Phytol. 136, 313-325.Google Scholar
  30. Lechowicz M J and Bell G 1991 The ecology and genetics of fitness in forest plants. II. Microspatial heterogeneity of the edaphic environment. J. Ecol. 79, 687-696.Google Scholar
  31. Magurran A E 1988 Ecological Diversity and its Measurement. Croom Helm, London. Nantel P and Neumann P 1992 Ecology of ectomycorrhizal-basidiomycete communities on a local vegetation gradient. Ecology 73(1), 99-117.Google Scholar
  32. Oliver M A and Badr I 1995 Determining the spatial scale of variation in soil radon concentration. Math. Geol. 27, 893-922.Google Scholar
  33. Peter M, Ayer F, Egli S and Honegger R 2001a Above-and below-ground community structure of ectomycorrhizal fungi in three Norway spruce (Picea abies) stands in Switzerland. Can. J. Bot. 79, 1134-1151.Google Scholar
  34. Peter M, Ayer F and Egli S 2001b Nitrogen addition in Norway spruce stand altered macromycete sporocarp production and below-ground ectomycorrhizal species composition measured by PCR-RFLP analysis of the ribosomal ITS-region. New Phytol. 149, 311-326.Google Scholar
  35. Read D J 1998 Plants on the web. Nature 396, 69-72.Google Scholar
  36. Redecker D, Szaro T M, Bowman R J and Bruns T D 2001 Small genets of Lactarius xanthogalactus, Russula cremoricolor and Amanita francheti in late stage ectomycorrhizal successions. Mol. Ecol. 10, 1025-1034.Google Scholar
  37. Sawyer N A, Chambers S M and Cairney J W G 1999 Molecular investigation of genet distribution and genetic variation of Cortinarius rotundisporus in eastern Australian sclerophyll forests. New Phytol. 142, 561-568.Google Scholar
  38. Sokal R R and Rohlf F J 1996 Introduction to Biostatistics. W.H. Freeman and Company, New York. 363 p.Google Scholar
  39. Stendell E R, Horton T R and Bruns T D 1999 Early effects of prescribed fire on the structure of the ectomycorrhizal fungus community in a Sierra Nevada ponderosa pine forest. Mycol. Res. 103, 1353-1359.Google Scholar
  40. Taylor A F S, Martin F and Read D J 2000 Fungal diversity in ectomycorrhizal communities of Norway spruce (Picea abies [L.] Karst.) and Beech (Fagus sylvatica L.) along north-south transects in Europe. In Ed. E-D. Schulze. Ecol. Stud. Vol. 142. pp 343-365. Springer-Verlag, Heidelberg.Google Scholar
  41. Taylor D L and Bruns T D 1999 Community structure of ectomycorrhizal fungi in a Pinus muricata forest: minimal overlap between the mature forest and resistant propagule communities. Mol. Ecol. 8, 1837-1850Google Scholar
  42. van der Heijden M G A, Klironomos J N, Ursic M, Moutoglis P, Streitwolf-Engel R, Boller T, Wiemken A and Sander I R1998 Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature 396, 69-72.Google Scholar
  43. Wallenda T and Kottke I 1998 Nitrogen deposition and ectomycorrhizas. New Phytol. 139, 169-187.Google Scholar
  44. White R E, Haigh R A and MacDuff J H 1987 Frequency distributions and spatially dependent variability of ammonium and nitrate concentrations in soil under grazed and ungrazed grassland. Fertil. Res. 11, 193-208.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Andy F. S. Taylor
    • 1
  1. 1.Department of Forest Mycology and PathologySwedish University of Agricultural SciencesUppsalaSweden

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