Inflammation

, Volume 23, Issue 6, pp 495–505

Mycoplasma fermentans—Induced Inflammatory Response of Astrocytes: Selective Modulation by Aminoguanidine, Thalidomide, Pentoxifylline and IL-10

  • Ruth Gallily
  • Marianne Kipper-Galperin
  • Talma Brenner
Article

DOI: 10.1023/A:1020234321879

Cite this article as:
Gallily, R., Kipper-Galperin, M. & Brenner, T. Inflammation (1999) 23: 495. doi:10.1023/A:1020234321879

Abstract

Exposure of primary rat glial cells, mostly astrocytes, to heat-inactivated Mycoplasma fermentans triggers the production of tumor necrosis factor α (TNFα) nitric oxide (NO) and prostaglandin E2 (PGE2). To attenuate the production of these proinflammatory mediators, four agents: aminoguanidine, pentoxifylline, thalidomide and IL-10 were added to astrocyte cultures. Aminoguanidine (1 and 3 mM), an inhibitor of inducible nitric oxide synthase (iNOS), suppressed the production of the three mediators. TNFα was the most sensitive to thalidomide, showing dose-response inhibition at concentrations of 20 μg/ml, 50 μg/ml and 250 μg/ml. PGE2 was affected only by concentrations of 50 μg/ml and 250 μg/ml, whereas NO responded solely to the highest amount of this inhibitor. The cytokine IL-10, at 10 U and 50 U, inhibited only TNFα production. Our results imply that selective suppression of proinflammatory mediators by various agents may prove feasible for amelioration of central nervous system inflammatory diseases.

Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • Ruth Gallily
    • 1
  • Marianne Kipper-Galperin
    • 2
  • Talma Brenner
    • 2
  1. 1.The Lautenberg Center for General and Tumor ImmunologyHadassah Medical School, Hebrew UniversityIsrael
  2. 2.Laboratory of Neuroimmunology, Department of NeurologyHadassah University HospitalJerusalemIsrael

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