Inflammation

, Volume 23, Issue 1, pp 1–13

Interleukin-4 and Interferon-γ Inhibit Prostaglandin Production by Interleukin-1β-Stimulated Human Periodontal Ligament Fibroblasts

  • K. Noguchi
  • M. Shitashige
  • H. Watanabe
  • S. Murota
  • I. Ishikawa
Article

Abstract

The purpose of the present study was to investigate the involvement of cyclooxygease-1 (COX-1) and cyclooxygenase-2 (COX-2) in prostaglandin (PG) production by human periodontal ligament (PDL) fibroblasts stimulated with a proinflammatory cytokine, inerleukin-1β (IL-1β), and to examine the effect of interleukin-4 (IL-4), a Th2 cytokine, and interferon-γ (IFN-γ), a Th1 cytokine, on PG production by the cells. IL-1β-stimulated PDL fibroblasts produced prostaglandin E2 (PGE2) in a time-dependent manner. Indomethacin, a non-selective COX-1/COX-2 inhibitor, and NS-398, a selective COX-2 inhibitor, completely inhibited PGE2 production by IL-1β-stimulated cells. Northern blot analysis showed that COX-2 mRNA was detected in IL-1β-stimulated PDL cells, although not detected in unstimulated cells, while expression of COX-1 mRNA was in the same extent in both the cells. Dexamethasone inhibited COX-2 mRNA expression, COX activity and PGE2 production in IL-1β-stimulated cells. IL-4 and IFN-γ suppressed PGE2 production by IL-1β-stimulated PDL fibroblasts, but COX activity enhanced by IL-1β treatment was significantly inhibited by IL-4, not by IFN-γ. Northern blot analysis showed that IL-4 depressed COX-2 mRNA expression with no effect on COX-1 mRNA expression. On the other hand, IFN-γ had no effect on expression of COX-1 and -2 mRNA. These data suggest that COX-2 is primarily responsible for PGE2 production by IL-1β-stimulated human PDL fibroblasts and that IL-4 inhibited PGE2 production by IL-1β-stimulated PDL fibroblasts through down-regulation of COX-2 expression, while IFN-γ suppressed the PGE2 production with no effect on COX-2 expression.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Plenum Publishing Corporation 1999

Authors and Affiliations

  • K. Noguchi
    • 1
  • M. Shitashige
    • 2
  • H. Watanabe
    • 1
  • S. Murota
    • 2
  • I. Ishikawa
    • 1
  1. 1.Department of Periodontology, Graduate School, Faculty of DentistryTokyo Medical and Dental UniversityBunkyo-ku, TokyoJapan
  2. 2.Department of Physiological Chemistry, Graduate School, Faculty of DentistryTokyo Medical and Dental UniversityBunkyo-ku, TokyoJapan

Personalised recommendations