Novel male-specific molecular markers (MADC5, MADC6) in hemp
- 389 Downloads
Decamer RAPD primers were tested on dioecious and monoecious hemp cultivars to identify sex-specific molecular markers. Two primers (OPD05 and UBC354) generated specific bands in male plants. These two DNA fragments were isolated, cloned and sequenced. Both markers proved to be unique, since no sequence with significant homology to OPD05961 and UBC354151 markers were found in databases. These markers were named MADC3 (OPD05961) and MADC4 (UBC354151) (Male-Associated DNA from Cannabis sativa). The markers were converted into sequence-characterized amplified region (SCAR) markers. The SCAR markers correlated with the sex of the segregating F2 population and proved the tight linkage to the male phenotype. Results of F2 plant population analysis suggest these markers are to be linked to the Y chromosome.
Unable to display preview. Download preview PDF.
- Bócsa, I., 1998. Genetic improvement: conventional approaches. In: P. Ranalli (Ed.), Advances in Hemp Research, pp. 153–184. Food Products press, An Imprint of The Haworth Press, Inc. New York.Google Scholar
- Clarke, R.K., 1997. Hanf: Botanik, Anbau Vermehrung und Züchtung. AT Verlag, Aarau, Schweiz.Google Scholar
- Hirata, K., 1924. Cytological basis of the sex determination in Cannabis sativa L. Jap J Genet 4: 198–201.Google Scholar
- Jagadish, V., J. Robertson & A. Gibbs, 1996. RAPD analysis distinguishes Cannabis sativa samples from different sources. For Sci Int 79: 113–121.Google Scholar
- Mandolino, G., A. Carboni, S. Forapani & P. Ranalli, 1998. DNA markers associated with sex phenotype in hemp (Cannabis sativa L). Proc of ‘Blast Fibrous Plants Today and Tomorrow’ St. Petersburg, September 28-30: 197–201, Institute of Natural Fibres (Poland), special edition, 1998/2.Google Scholar
- Michelmore, R.W., I. Paran & R.V. Kesseli, 1991. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828–9832.PubMedCrossRefGoogle Scholar
- Migal, N.D., 1986. Genetic determination of sex in hemp. II. Sexual mutations and the general theory of genotypic control of sex in hemp. Genetika USSR 22: 829–837.Google Scholar
- Sakamoto, K., Y. Akiyama, K. Fukui, H. Kamada & S. Satoh, 1998. Characterization, genom sizes and morfology of sex chromosomes in hemp (Cannabis sativa L). Cytologia 63: 459–464.Google Scholar
- Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.Google Scholar
- Warmke, H.E. & H. Davidson, 1944. Poliploid investigation. Yearbook of the Carnegie Institution of Washington 43: 135–139.Google Scholar
- Yamada, I., 1943. The sex-chromosome of Cannabis sativa L. Seiken Ziho 2: 64–68.Google Scholar
- Yampolsky, C. & H., Yampolsky, 1922. Distribution of sex forms in the phanerogamic flora. Bibl Genet 3: 1–62.Google Scholar