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Apoptosis

, Volume 7, Issue 5, pp 407–412 | Cite as

Didemnin B induces apoptosis in proliferating but not resting peripheral blood mononuclear cells

  • M. A. Baker
  • D. R. Grubb
  • A. Lawen
Article

Abstract

We have previously shown that didemnin B, a branched cyclic depsipeptide composed of seven amino acids and two hydroxy acids, can induce rapid and complete apoptosis in HL-60 cells (Grubb, D.R. et al. (1995) Biochem. Biophys. Res. Commun. 215, 1130–1136). We now report that didemnin B can induce apoptosis in a wide range of transformed cell lines. Resting normal lymphocytes, however, are apparently unaffected by exposure to the drug. To investigate whether cell transformation, and/or cell proliferation is necessary for didemnin B to induce apoptosis, we examined the effect of didemnin B on freshly harvested human lymphocytes before and after stimulation with concanavalin A. Didemnin B induced apoptosis in normal lymphocytes only after mitogenic stimulation and therefore warrants further examination for its potential as a chemotherapeutic agent, especially for treatment of leukemia.

apoptosis cell proliferation chemotherapy concanavalin A didemnin B leukemia 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • M. A. Baker
    • 1
  • D. R. Grubb
    • 1
  • A. Lawen
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, School of Biomedical SciencesMonash UniversityAustralia

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